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Review

System-Based Approaches to Delineate the Antiviral Innate Immune Landscape

1
School of Medicine, Institute of Virology, Technical University of Munich, 81675 Munich, Germany
2
German Center for Infection Research (DZIF), Munich Partner Site, 80538 Munich, Germany
*
Author to whom correspondence should be addressed.
Viruses 2020, 12(10), 1196; https://doi.org/10.3390/v12101196
Received: 25 September 2020 / Revised: 19 October 2020 / Accepted: 19 October 2020 / Published: 21 October 2020
(This article belongs to the Special Issue Innate Immune Sensing of Viruses and Viral Evasion)
Viruses pose substantial challenges for society, economy, healthcare systems, and research. Their distinctive pathologies are based on specific interactions with cellular factors. In order to develop new antiviral treatments, it is of central importance to understand how viruses interact with their host and how infected cells react to the virus on a molecular level. Invading viruses are commonly sensed by components of the innate immune system, which is composed of a highly effective yet complex network of proteins that, in most cases, mediate efficient virus inhibition. Central to this process is the activity of interferons and other cytokines that coordinate the antiviral response. So far, numerous methods have been used to identify how viruses interact with cellular processes and revealed that the innate immune response is highly complex and involves interferon-stimulated genes and their binding partners as functional factors. Novel approaches and careful experimental design, combined with large-scale, high-throughput methods and cutting-edge analysis pipelines, have to be utilized to delineate the antiviral innate immune landscape at a global level. In this review, we describe different currently used screening approaches, how they contributed to our knowledge on virus–host interactions, and essential considerations that have to be taken into account when planning such experiments. View Full-Text
Keywords: innate immunity; virus–host interactions; interferon-stimulated genes; genetic screens; gene regulation; knockout; overexpression innate immunity; virus–host interactions; interferon-stimulated genes; genetic screens; gene regulation; knockout; overexpression
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MDPI and ACS Style

Krey, K.; Babnis, A.W.; Pichlmair, A. System-Based Approaches to Delineate the Antiviral Innate Immune Landscape. Viruses 2020, 12, 1196. https://doi.org/10.3390/v12101196

AMA Style

Krey K, Babnis AW, Pichlmair A. System-Based Approaches to Delineate the Antiviral Innate Immune Landscape. Viruses. 2020; 12(10):1196. https://doi.org/10.3390/v12101196

Chicago/Turabian Style

Krey, Karsten, Aleksandra W. Babnis, and Andreas Pichlmair. 2020. "System-Based Approaches to Delineate the Antiviral Innate Immune Landscape" Viruses 12, no. 10: 1196. https://doi.org/10.3390/v12101196

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