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Open AccessArticle

A Cell-Based Capture Assay for Rapid Virus Detection

The Department of Infectious Diseases, Israel Institute for Biological Research, Ness-Ziona 74100, Israel
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Viruses 2020, 12(10), 1165; https://doi.org/10.3390/v12101165
Received: 27 August 2020 / Revised: 8 October 2020 / Accepted: 13 October 2020 / Published: 15 October 2020
(This article belongs to the Section Animal Viruses)
Routine methods for virus detection in clinical specimens rely on a variety of sensitive methods, such as genetic, cell culture and immuno-based assays. It is imperative that the detection assays would be reliable, reproducible, sensitive and rapid. Isolation of viruses from clinical samples is crucial for deeper virus identification and analysis. Here we introduce a rapid cell-based assay for isolation and detection of viruses. As a proof of concept several model viruses including West Nile Virus (WNV), Modified Vaccinia Ankara (MVA) and Adenovirus were chosen. Suspended Vero cells were employed to capture the viruses following specific antibody labeling which enables their detection by flow cytometry and immuno-fluorescence microscopy assays. Using flow cytometry, a dose response analysis was performed in which 3.6e4 pfu/mL and 1e6 pfu/mL of MVA and WNV could be detected within two hours, respectively. When spiked to commercial pooled human serum, detection sensitivity was slightly reduced to 3e6 pfu/mL for WNV, but remained essentially the same for MVA. In conclusion, the study demonstrates a robust and rapid methodology for virus detection using flow cytometry and fluorescence microscopy. We propose that this proof of concept may prove useful in identifying future pathogens. View Full-Text
Keywords: immuno-fluorescence assay; flow cytometry; West Nile Virus; Modified Vaccinia Ankara; Adenovirus; transmission electron microscopy immuno-fluorescence assay; flow cytometry; West Nile Virus; Modified Vaccinia Ankara; Adenovirus; transmission electron microscopy
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MDPI and ACS Style

Milrot, E.; Makdasi, E.; Politi, B.; Israely, T.; Laskar, O. A Cell-Based Capture Assay for Rapid Virus Detection. Viruses 2020, 12, 1165.

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