Targeting Human Parainfluenza Virus Type-1 Haemagglutinin-Neuraminidase with Mechanism-Based Inhibitors
Abstract
:1. Introduction
2. Material and Methods
2.1. hPIV-1 HN Inhibitors
2.2. Cells and Viruses
2.3. Neuraminidase Inhibition Assay
2.4. Haemagglutination Inhibition Assay
2.5. In Situ ELISA
2.6. Recombinant hPIV-1 HN Expression and Purification
2.7. 1H NMR Experiments
3. Results
3.1. hPIV-1 HN Neuraminidase Activity Characterisation and Blockade
3.2. Inhibition of hPIV-1 HN Haemagglutination and Neuraminidase Functions
3.3. In Vitro Viral Replication Inhibition
4. Discussion
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Eveno, T.; Dirr, L.; El-Deeb, I.M.; Guillon, P.; von Itzstein, M. Targeting Human Parainfluenza Virus Type-1 Haemagglutinin-Neuraminidase with Mechanism-Based Inhibitors. Viruses 2019, 11, 417. https://doi.org/10.3390/v11050417
Eveno T, Dirr L, El-Deeb IM, Guillon P, von Itzstein M. Targeting Human Parainfluenza Virus Type-1 Haemagglutinin-Neuraminidase with Mechanism-Based Inhibitors. Viruses. 2019; 11(5):417. https://doi.org/10.3390/v11050417
Chicago/Turabian StyleEveno, Tanguy, Larissa Dirr, Ibrahim M. El-Deeb, Patrice Guillon, and Mark von Itzstein. 2019. "Targeting Human Parainfluenza Virus Type-1 Haemagglutinin-Neuraminidase with Mechanism-Based Inhibitors" Viruses 11, no. 5: 417. https://doi.org/10.3390/v11050417