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Open AccessArticle

High Diversity and Novel Enteric Viruses in Fecal Viromes of Healthy Wild and Captive Thai Cynomolgus Macaques (Macaca fascicularis)

1
Vitalant Research Institute, San Francisco, CA 94118, USA
2
Department of Biochemistry, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand
3
Department of Laboratory Medicine, University of California San Francisco, San Francisco, CA 9413, USA
4
National Primate Research Center-Chulalongkorn University, Saraburi 18110, Thailand
5
Center of Excellence in Systems Biology, Chulalongkorn University (CUSB), Bangkok 10330, Thailand
*
Author to whom correspondence should be addressed.
Viruses 2019, 11(10), 971; https://doi.org/10.3390/v11100971
Received: 9 September 2019 / Revised: 6 October 2019 / Accepted: 7 October 2019 / Published: 22 October 2019
(This article belongs to the Section Animal Viruses)
Cynomolgus macaques are common across South East Asian countries including Thailand. The National Primate Research Center of Thailand, Chulalongkorn University (NPRCT-CU) captures wild-borne cynomolgus macaque for research use. Limited information is available on the enteric viruses and possible zoonotic infections into or from cynomolgus macaques. We characterized and compare the fecal virome of two populations; healthy wild-originated captive cynomolgus macaques (n = 43) reared in NPRCT-CU and healthy wild cynomolgus macaques (n = 35). Over 90% of recognized viral sequence reads amplified from feces were from bacterial viruses. Viruses from seven families of mammalian viruses were also detected (Parvoviridae, Anelloviridae, Picornaviridae, Adenoviridae, Papillomaviridae, Herpesviridae, and Caliciviridae). The genomes of a member of a new picornavirus genus we named Mafapivirus, a primate chapparvovirus, and a circular Rep-encoding single-strand (CRESS) DNA virus were also characterized. Higher abundance of CRESS DNA viruses of unknown tropism and invertebrate-tropic ambidensovirus were detected in wild versus captive macaques likely reflecting dietary differences. Short term rearing in captivity did not have a pronounced effect on the diversity of mammalian viruses of wild cynomolgus macaques. This study is the first report of the fecal virome of cynomolgus macaques, non-human primates frequently used in biomedical research and vaccination studies. View Full-Text
Keywords: long-tailed macaque; virome; high-throughput sequencing; viral discovery long-tailed macaque; virome; high-throughput sequencing; viral discovery
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Sawaswong, V.; Fahsbender, E.; Altan, E.; Kemthong, T.; Deng, X.; Malaivijitnond, S.; Payungporn, S.; Delwart, E. High Diversity and Novel Enteric Viruses in Fecal Viromes of Healthy Wild and Captive Thai Cynomolgus Macaques (Macaca fascicularis). Viruses 2019, 11, 971.

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