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Viruses 2018, 10(6), 324; https://doi.org/10.3390/v10060324

Production of Bacteriophages by Listeria Cells Entrapped in Organic Polymers

1
Département de Biochimie, de Microbiologie et de Bio-Informatique, Faculté des Sciences et de Génie, Université Laval, Québec, QC G1V OA6, Canada
2
Département des Sciences des Aliments, Faculté des Sciences de L’agriculture et de L’alimentation, Université Laval, Québec, QC G1V OA6, Canada
3
Félix d’Hérelle Reference Center for Bacterial Viruses and GREB, Faculté de Médecine Dentaire, Université Laval, Québec, QC G1V OA6, Canada
4
ETH Zurich, Institute of Food, Nutrition and Health, Schmelzbergstrasse, 7CH-8092 Zürich, Switzerland
*
Author to whom correspondence should be addressed.
Received: 13 May 2018 / Revised: 7 June 2018 / Accepted: 8 June 2018 / Published: 13 June 2018
(This article belongs to the Special Issue Hurdles for Phage Therapy (PT) to Become a Reality)
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Abstract

Applications for bacteriophages as antimicrobial agents are increasing. The industrial use of these bacterial viruses requires the production of large amounts of suitable strictly lytic phages, particularly for food and agricultural applications. This work describes a new approach for phage production. Phages H387 (Siphoviridae) and A511 (Myoviridae) were propagated separately using Listeria ivanovii host cells immobilised in alginate beads. The same batch of alginate beads could be used for four successive and efficient phage productions. This technique enables the production of large volumes of high-titer phage lysates in continuous or semi-continuous (fed-batch) cultures. View Full-Text
Keywords: Listeria ivanovii; bacteriophages; alginate; production; disinfection; phagodisinfection Listeria ivanovii; bacteriophages; alginate; production; disinfection; phagodisinfection
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Roy, B.; Philippe, C.; Loessner, M.J.; Goulet, J.; Moineau, S. Production of Bacteriophages by Listeria Cells Entrapped in Organic Polymers. Viruses 2018, 10, 324.

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