A Simple and Robust Approach for Evaluation of Antivirals Using a Recombinant Influenza Virus Expressing Gaussia Luciferase
Abstract
:1. Introduction
2. Materials and Methods
2.1. Cells and Plasmids
2.2. Virus Rescue and Titration
2.3. Multicycle Replication Assay
2.4. Gaussia Luciferase Assay
2.5. Antiviral Determination
2.6. Mouse Infections
2.7. Haematoxylin and Eosin Staining
3. Results
3.1. In Vitro Properties of PR8–NS1–Gluc
3.2. PR8–NS1–Gluc Virus Is Pathogenic in Mice
3.3. Dynamics of Luciferase Expression in the Lungs of the Infected Mice
3.4. Evaluation of Antivirals In Vitro
3.5. Evaluation of Antiviral Interventions in a Mouse Model
4. Discussion
Supplementary Materials
Author Contributions
Acknowledgments
Conflicts of Interest
References
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Li, P.; Cui, Q.; Wang, L.; Zhao, X.; Zhang, Y.; Manicassamy, B.; Yang, Y.; Rong, L.; Du, R. A Simple and Robust Approach for Evaluation of Antivirals Using a Recombinant Influenza Virus Expressing Gaussia Luciferase. Viruses 2018, 10, 325. https://doi.org/10.3390/v10060325
Li P, Cui Q, Wang L, Zhao X, Zhang Y, Manicassamy B, Yang Y, Rong L, Du R. A Simple and Robust Approach for Evaluation of Antivirals Using a Recombinant Influenza Virus Expressing Gaussia Luciferase. Viruses. 2018; 10(6):325. https://doi.org/10.3390/v10060325
Chicago/Turabian StyleLi, Ping, Qinghua Cui, Lin Wang, Xiujuan Zhao, Yingying Zhang, Balaji Manicassamy, Yong Yang, Lijun Rong, and Ruikun Du. 2018. "A Simple and Robust Approach for Evaluation of Antivirals Using a Recombinant Influenza Virus Expressing Gaussia Luciferase" Viruses 10, no. 6: 325. https://doi.org/10.3390/v10060325