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Viruses 2018, 10(5), 265; https://doi.org/10.3390/v10050265

Inhibition of Human Immunodeficiency Virus Type 1 Entry by a Keggin Polyoxometalate

1,†
,
2,†
,
1,†
,
1
,
1
,
1,†,* , 1
,
2,†,* , 1
and
2,†,*
1
College of Life Science and Bioengineering, Beijing University of Technology, Beijing 100124, China
2
National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China
These authors contributed equally to this work.
*
Authors to whom correspondence should be addressed.
Received: 20 March 2018 / Revised: 11 May 2018 / Accepted: 14 May 2018 / Published: 16 May 2018
(This article belongs to the Section Antivirals & Vaccines)
Full-Text   |   PDF [1874 KB, uploaded 16 May 2018]   |  

Abstract

Here, we report the anti-human immunodeficiency virus (HIV) potency and underlying mechanisms of a Keggin polyoxometalate (PT-1, K6HPTi2W10O40). Our findings showed that PT-1 exhibited highly potent effects against a diverse group of HIV type 1 (HIV-1) strains and displayed low cytotoxicity and genotoxicity. The time-addition assay revealed that PT-1 acted at an early stage of infection, and these findings were supported by the observation that PT-1 had more potency against Env-pseudotyped virus than vesicular stomatitis virus glycoprotein (VSVG) pseudotyped virus. Surface plasmon resonance binding assays and flow cytometry analysis showed that PT-1 blocked the gp120 binding site in the CD4 receptor. Moreover, PT-1 bound directly to gp41 NHR (N36 peptide), thereby interrupting the core bundle formation of gp41. In conclusion, our data suggested that PT-1 may be developed as a new anti-HIV-1 agent through its effects on entry inhibition. View Full-Text
Keywords: human immunodeficiency virus type 1; Keggin polyoxometalate; entry inhibition; CD4; gp41 NHR human immunodeficiency virus type 1; Keggin polyoxometalate; entry inhibition; CD4; gp41 NHR
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Wang, X.; Wang, J.; Zhang, W.; Li, B.; Zhu, Y.; Hu, Q.; Yang, Y.; Zhang, X.; Yan, H.; Zeng, Y. Inhibition of Human Immunodeficiency Virus Type 1 Entry by a Keggin Polyoxometalate. Viruses 2018, 10, 265.

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