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Open AccessArticle

Non-Targeted Metabolomics Reveals Patterns of Metabolic Changes during Poplar Seed Germination

1
State Key Laboratory of Tree Genetics and Breeding (Northeast Forestry University), School of Forestry, Northeast Forestry University, Harbin 150040, China
2
School of Forestry, Northeast Forestry University, Harbin 150040, China
3
College of Life Science, Northeast Forestry University, Harbin 150040, China
4
College of Life Science and Technology, Harbin Normal University, Harbin 150025, China
5
Key Laboratory of Forestry Plant Ecology of Ministry of Education, Northeast Forestry University, Harbin 150040, China
*
Author to whom correspondence should be addressed.
Forests 2019, 10(8), 659; https://doi.org/10.3390/f10080659
Received: 12 July 2019 / Revised: 30 July 2019 / Accepted: 2 August 2019 / Published: 6 August 2019
(This article belongs to the Special Issue Forest Genetic and Tree Improvement)
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PDF [3173 KB, uploaded 6 August 2019]
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Abstract

Research Highlights: This study was the first to use metabolomics techniques to investigate seed germination in poplar, a model woody plant. Our results lay a foundation for uncovering changes in metabolite levels during woody plant seed germination and for understanding the underlying mechanism. Background and Objectives: Poplar is a model woody plant. Because poplar can be easily propagated asexually, the molecular mechanism of poplar seed germination has not been well studied. However, long-term asexual reproduction of poplar leads to seedlings with weak resistance, high vulnerability to degradation, and reduced growth potential. Materials and Methods: The non-targeted metabolomics technique was used to analyze changing trends in metabolite contents during the poplar seed germination process. Results: We found that the number of differential metabolites increased as seed germination progressed. Metabolic pathway analysis of differential metabolites revealed that galactose metabolism and alanine, aspartate, and glutamate metabolism were significantly enriched during all germination periods. MapMan-based visual analysis of metabolic pathways of differential metabolites indicated that glutamine, glutamic acid, phenylalanine, arginine, and asparagine contents increased with germination time, with most metabolites related to glucose metabolism following similar trends. Contents of most metabolites related to the tricarboxylic acid cycle exhibited a fluctuating pattern. Conclusions: This study has revealed the major changes taking place in primary metabolite contents during poplar seed germination and has laid the foundation for elucidation of the molecular mechanism of poplar seed germination. View Full-Text
Keywords: poplar; seed germination; metabolite; non-targeted metabolomics poplar; seed germination; metabolite; non-targeted metabolomics
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Qu, C.; Chen, J.; Cao, L.; Teng, X.; Li, J.; Yang, C.; Zhang, X.; Zhang, Y.; Liu, G.; Xu, Z. Non-Targeted Metabolomics Reveals Patterns of Metabolic Changes during Poplar Seed Germination. Forests 2019, 10, 659.

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