The Beneficial Effects of Citrus kawachiensis Peel on Neurogenesis in the Hippocampus and Gut Microbiota Changes in a Chronic Unpredictable Mild Stress Mouse Model
Abstract
:1. Introduction
2. Materials and Methods
2.1. Sample Preparation
2.2. Experimental Schedule and CUMS
2.3. OFT
2.4. Western Blot Analysis
2.5. Immunofluorescence Staining
2.6. Next-Generation Sequencing
2.7. HPLC Analysis
2.8. Statistical Analysis
3. Results
3.1. Body Weight Changes and Behavioral Analyses
3.2. Western Blot and Immunofluorescent Analyses of the Hippocampus
3.3. Analysis of the Cecal Contents Using Next-Generation Sequencing and HPLC
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Acknowledgments
Conflicts of Interest
References
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Okuyama, S.; Kotani, M.; Ninomiya, F.; Sawamoto, A.; Fujitani, M.; Ano, Y.; Kishida, T.; Nakajima, M.; Furukawa, Y. The Beneficial Effects of Citrus kawachiensis Peel on Neurogenesis in the Hippocampus and Gut Microbiota Changes in a Chronic Unpredictable Mild Stress Mouse Model. Nutraceuticals 2022, 2, 91-101. https://doi.org/10.3390/nutraceuticals2020007
Okuyama S, Kotani M, Ninomiya F, Sawamoto A, Fujitani M, Ano Y, Kishida T, Nakajima M, Furukawa Y. The Beneficial Effects of Citrus kawachiensis Peel on Neurogenesis in the Hippocampus and Gut Microbiota Changes in a Chronic Unpredictable Mild Stress Mouse Model. Nutraceuticals. 2022; 2(2):91-101. https://doi.org/10.3390/nutraceuticals2020007
Chicago/Turabian StyleOkuyama, Satoshi, Maho Kotani, Fuga Ninomiya, Atsushi Sawamoto, Mina Fujitani, Yoshitaka Ano, Taro Kishida, Mitsunari Nakajima, and Yoshiko Furukawa. 2022. "The Beneficial Effects of Citrus kawachiensis Peel on Neurogenesis in the Hippocampus and Gut Microbiota Changes in a Chronic Unpredictable Mild Stress Mouse Model" Nutraceuticals 2, no. 2: 91-101. https://doi.org/10.3390/nutraceuticals2020007