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Int. J. Environ. Res. Public Health 2019, 16(3), 474; https://doi.org/10.3390/ijerph16030474

Oxidative Stress, Induced by Sub-Lethal Doses of BDE 209, Promotes Energy Management and Cell Cycle Modulation in the Marine Fish Cell Line SAF-1

1
Laboratory of Marine biochemistry and ecotoxicology, Department of Earth and Marine Science DISTEM, University of Palermo, Via Barlotta 4, 91100 Trapani, Italy
2
Fish Innate Immune System Group, Department of Cell Biology and Histology, Faculty of Biology, Campus Regional de Excelencia Internacional “Campus Mare Nostrum”, University of Murcia, 30100 Murcia, Spain
3
Marine Biology Institute, Consorzio Universitario della Provincia di Trapani, Via Barlotta 4, 91100 Trapani, Italy
*
Author to whom correspondence should be addressed.
Received: 2 January 2019 / Revised: 1 February 2019 / Accepted: 2 February 2019 / Published: 6 February 2019
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Abstract

The effects of sub-lethal doses of polybrominated diphenyl ether (PBDE)-209 in terms of toxicity, oxidative stress, and biomarkers were evaluated in the Sparus aurata fibroblast cell line (SAF-1). Vitality and oxidative stress status were studied after incubation with PBDE for 72 h. Concomitantly, the quantification of proteins related to cell cycle and DNA repair (p53), cell proliferation (extracellular signal–regulated kinase 1 (ERK1)), energetic restriction (hypoxia-inducible factor 1 (HIF1)), and redox status (Nuclear factor erythroid 2–related factor 2 (NRF2)) was also determined after prolonged exposure (7–15 days) by immunoblotting. Our results demonstrated that rising concentrations of PBDEs exposure-induced oxidative stress, and that this event modulates different cell pathways related to cell cycle, cell signaling, and energetic balance in the long term, indicating the negative impact of sub-lethal dose exposure to cell homeostasis. View Full-Text
Keywords: PBDE-209; Sparus aurata fibroblast; oxidative stress; biomarkers PBDE-209; Sparus aurata fibroblast; oxidative stress; biomarkers
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Espinosa Ruiz, C.; Manuguerra, S.; Cuesta, A.; Santulli, A.; Messina, C.M. Oxidative Stress, Induced by Sub-Lethal Doses of BDE 209, Promotes Energy Management and Cell Cycle Modulation in the Marine Fish Cell Line SAF-1. Int. J. Environ. Res. Public Health 2019, 16, 474.

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