Quantitative Ultra-Performance Liquid Chromatography Tandem Mass Spectrometry Method for Comparison of Prochloraz Residue on Garlic Sprouts after Soaking and Spraying Treatment
Abstract
:1. Introduction
2. Materials and Methods
2.1. Reagents
2.2. Postharvest Treatment and Storage
2.3. Sample Preparation
2.3.1. Sample Extraction
2.3.2. Purification
2.3.3. Method Validation
2.4. UPLC-MS/MS Conditions
3. Results and Discussion
3.1. Limit of Detection and LOQ
3.2. Linearity
3.3. Recovery Study
3.4. Actual Sample
4. Conclusions
Author Contributions
Acknowledgments
Conflicts of Interest
References
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Compound | Transitions | Dwell Time (s) | Cone Voltage (V) | Collision Energy (eV) |
---|---|---|---|---|
Prochloraz | Quantification ion: 376 > 308 | 0.008 | 20 | 15 |
Confirmation ion: 376 > 266 | 20 | 15 |
Pesticide | ME (%) | tR (min) | Linear Range (μg/kg) | Linear Regression Equation | Linearity |
---|---|---|---|---|---|
Prochloraz | 5.8 | 2.20 | 5–500 | Y = 0.9898X − 1.2624 | 0.9983 |
Pesticide | LOD (μg/kg) | LOQ (μg/kg) | Concentration (μg/kg) | Measured ± SD (μg/kg) | Recovery (%) | RSD (%) |
---|---|---|---|---|---|---|
Prochloraz | 0.0166 | 0.0499 | 5 | 4.7 ± 0.46 | 94.8 | 9.7 |
50 | 44.2 ± 1.26 | 88.4 | 2.8 | |||
500 | 447.1 ± 11.69 | 89.4 | 2.6 |
Sampling City | Sampling Time | Soaking (mg/kg) | Spraying (mg/kg) | ||
---|---|---|---|---|---|
Whole Plant (Measured ± SD) | Stems (Measured ± SD) | Whole Plant (Measured ± SD) | Stems (Measured ± SD) | ||
Pingdu | 1 June | 25.14 ± 1.20 | 1.12 ± 0.07 | 5.93 ± 0.36 | 1.11 ± 0.06 |
29 June | 22.72 ± 0.94 | 0.80 ± 0.03 | 2.23 ± 0.18 | 0.12 ± 0.01 | |
30 July | 21.86 ± 1.13 | 0.58 ± 0.04 | 1.85 ± 0.12 | 0.01 ± 0.001 | |
Laixi | 1 June | 20.12 ± 0.86 | 1.62 ± 0.05 | 7.89 ± 0.54 | 1.29 ± 0.09 |
29 June | 18.70 ± 0.42 | 1.13 ± 0.09 | 5.04 ± 0.30 | 0.15 ± 0.01 | |
30 July | 15.76 ± 1.06 | 1.04 ± 0.06 | 4.08 ± 0.20 | 0.03 ± 0.003 |
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Fang, Q.; Ding, C.; Dong, Z.; Guan, S.; Wu, R.; Wu, X.; Hua, R.; Cao, H. Quantitative Ultra-Performance Liquid Chromatography Tandem Mass Spectrometry Method for Comparison of Prochloraz Residue on Garlic Sprouts after Soaking and Spraying Treatment. Int. J. Environ. Res. Public Health 2018, 15, 1552. https://doi.org/10.3390/ijerph15071552
Fang Q, Ding C, Dong Z, Guan S, Wu R, Wu X, Hua R, Cao H. Quantitative Ultra-Performance Liquid Chromatography Tandem Mass Spectrometry Method for Comparison of Prochloraz Residue on Garlic Sprouts after Soaking and Spraying Treatment. International Journal of Environmental Research and Public Health. 2018; 15(7):1552. https://doi.org/10.3390/ijerph15071552
Chicago/Turabian StyleFang, Qingkui, Chenchun Ding, Zhan Dong, Shuai Guan, Ruifeng Wu, Xiangwei Wu, Rimao Hua, and Haiqun Cao. 2018. "Quantitative Ultra-Performance Liquid Chromatography Tandem Mass Spectrometry Method for Comparison of Prochloraz Residue on Garlic Sprouts after Soaking and Spraying Treatment" International Journal of Environmental Research and Public Health 15, no. 7: 1552. https://doi.org/10.3390/ijerph15071552