Next Article in Journal
Comparative Evaluation of Different Chitosan Species and Derivatives as Candidate Biomaterials for Oxygen-Loaded Nanodroplet Formulations to Treat Chronic Wounds
Next Article in Special Issue
Secondary Metabolites from the Marine Sponges of the Genus Petrosia: A Literature Review of 43 Years of Research
Previous Article in Journal
Antimicrobial Activity of Chitosan Oligosaccharides with Special Attention to Antiparasitic Potential
Previous Article in Special Issue
Recent Advances in Marine-Based Nutraceuticals and Their Health Benefits
 
 
Article

Using Bacillus subtilis as a Host Cell to Express an Antimicrobial Peptide from the Marine Chordate Ciona intestinalis

1
Institute of Biomedical Sciences, Mackay Medical College, New Taipei City 25245, Taiwan
2
Institute of Molecular and Cellular Biology, National Taiwan University, Taipei 10617, Taiwan
3
Liver Disease Prevention and Treatment Research Foundation, Taipei 10047, Taiwan
4
Department of Life Science, Fu Jen Catholic University, New Taipei 20206, Taiwan
*
Author to whom correspondence should be addressed.
Academic Editor: Vida Šimat
Mar. Drugs 2021, 19(2), 111; https://doi.org/10.3390/md19020111
Received: 3 February 2021 / Revised: 8 February 2021 / Accepted: 8 February 2021 / Published: 12 February 2021
(This article belongs to the Special Issue Nutraceuticals and Pharmaceuticals from Marine Fish and Invertebrates)
Ciona molecule against microbes-A24 (CiMAM) isolated from the marine chordate Ciona intestinalis is an antimicrobial peptide. To generate CiMAM-expressing transgenic Bacillus subtilis, we constructed a plasmid expressing recombinant CiMAM (rCiMAM) and introduced it into B. subtilis. Transgenic strains C117 and C166 were selected since they were able to highly and stably express rCiMAM. We studied the bactericidal activity of pepsin-digested extracts from rCiMAM-expressing strains against freshwater and euryhaline pathogens that commonly occur in aquaculture ponds and found no difference from that of lactoferricin-expressing strains. The bactericidal activity of 1-μL aliquot from a total 5.5 mL extracted from 5 mL of cultured C117 (1.45 × 108 CFU·mL−1) and C166 (2.17 × 108 CFU·mL−1) against halophilic bacteria was equivalent to the efficacy of 57.06 and 32.35 ng of Tetracycline against Vibrio natriegens, 47.07 and 25.2 ng against V. parahaemolyticus, and 58.17 and 36.55 ng against V. alginolyticus, respectively, indicating higher bactericidal activity of pepsin-extracts from rCiMAM-containing strains against halophilic bacteria compared to that from lactoferricin-containing strains. Since the antibacterial activity of rCiMAM-expressing B. subtilis strains shows higher competence against halophilic pathogens compared to that against freshwater and euryhaline pathogens, these strains are promising candidates to protect marine fish and shellfish from halophilic bacterial infection. View Full-Text
Keywords: CiMAM; antimicrobial peptide; lactoferricin; transgenic line; bactericidal activity CiMAM; antimicrobial peptide; lactoferricin; transgenic line; bactericidal activity
Show Figures

Figure 1

MDPI and ACS Style

Lee, B.-C.; Tsai, J.-C.; Lin, C.-Y.; Hung, C.-W.; Sheu, J.-C.; Tsai, H.-J. Using Bacillus subtilis as a Host Cell to Express an Antimicrobial Peptide from the Marine Chordate Ciona intestinalis. Mar. Drugs 2021, 19, 111. https://doi.org/10.3390/md19020111

AMA Style

Lee B-C, Tsai J-C, Lin C-Y, Hung C-W, Sheu J-C, Tsai H-J. Using Bacillus subtilis as a Host Cell to Express an Antimicrobial Peptide from the Marine Chordate Ciona intestinalis. Marine Drugs. 2021; 19(2):111. https://doi.org/10.3390/md19020111

Chicago/Turabian Style

Lee, Bing-Chang, Jui-Che Tsai, Cheng-Yung Lin, Chun-Wei Hung, Jin-Chuan Sheu, and Huai-Jen Tsai. 2021. "Using Bacillus subtilis as a Host Cell to Express an Antimicrobial Peptide from the Marine Chordate Ciona intestinalis" Marine Drugs 19, no. 2: 111. https://doi.org/10.3390/md19020111

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop