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Open AccessArticle

Effect of Chitosan as a Cross-Linker on Matrix Metalloproteinase Activity and Bond Stability with Different Adhesive Systems

Area of Stomatology, Health Sciences Faculty, King Juan Carlos University, Avda. de Atenas, s/n. 28922 Alcorcón, Madrid, Spain
Department of Biomedical and Neuromotor Sciences, DIBINEM, University of Bologna, Alma Mater Studiorum, Via San Vitale 59, 40125 Bologna, Italy
Department of Restorative Dentistry, School of Dentistry, University of São Paulo, São Paulo 14040-904, Brazil
Department of Operative Dentistry, College of Dentistry, University of Iowa, 801 Newton Rd, Iowa City, IA 52242, USA
School of Dentistry, Faculty of Medicine, University of Novi Sad, Hajduk Veljkova 3, 21000 Novi Sad, Serbia
Department of Endodontics, The Dental College of Georgia, Augusta University, 1430 John Wesley Gilbert Drive, Augusta, GA 30912, USA
Author to whom correspondence should be addressed.
Mar. Drugs 2020, 18(5), 263;
Received: 21 April 2020 / Revised: 11 May 2020 / Accepted: 13 May 2020 / Published: 18 May 2020
The aim of the present study was to evaluate the effect of 0.1% chitosan (Ch) solution as an additional primer on the mechanical durability and enzymatic activity on dentine using an etch-and-rinse (E&R) adhesive and a universal self-etch (SE) adhesive. Microtensile bond strength and interfacial nanoleakage expression of the bonded interfaces for all adhesives (with or without pretreatment with 0.1% Ch solution for 1 min and air-dried for 5 s) were analyzed immediately and after 10,000 thermocycles. Zymograms of protein extracts from human dentine powder incubated with Optibond FL and Scotchbond Universal on untreated or Ch-treated dentine were obtained to examine dentine matrix metalloproteinase (MMP) activities. The use of 0.1% Ch solution as an additional primer in conjunction with the E&R or SE adhesive did not appear to have influenced the immediate bond strength (T0) or bond strength after thermocycling (T1). Zymography showed a reduction in MMP activities only for mineralized and demineralized dentine powder after the application of Ch. Application of 0.1% Ch solution does not increase the longevity of resin–dentine bonds. Nonetheless, the procedure appears to be proficient in reducing dentine MMP activities within groups without adhesive treatments. Further studies are required to comprehend the cross-linking of Ch with dentine collagen. View Full-Text
Keywords: chitosan; matrix metalloproteinases; bond strength; cross-linkers; zymography chitosan; matrix metalloproteinases; bond strength; cross-linkers; zymography
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Baena, E.; Cunha, S.R.; Maravić, T.; Comba, A.; Paganelli, F.; Alessandri-Bonetti, G.; Ceballos, L.; Tay, F.R.; Breschi, L.; Mazzoni, A. Effect of Chitosan as a Cross-Linker on Matrix Metalloproteinase Activity and Bond Stability with Different Adhesive Systems. Mar. Drugs 2020, 18, 263.

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