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Open AccessArticle

Application of Gelatin Decorated with Allura Red as Resonance Rayleigh Scattering Sensor to Detect Chito-Oligosaccharides

by Weiling Zou 1, Zijun Sun 1, Zhengquan Su 2,* and Yan Bai 1,*
1
School of Public Health, Guangdong Pharmaceutical University, Guangzhou 510310, China
2
Guangdong Engineering Research Center of Natural Products and New Drugs, Guangdong Provincial University Engineering Technology Research Center of Natural Products and Drugs, Guangdong Pharmaceutical University, Guangzhou 510006, China
*
Authors to whom correspondence should be addressed.
Mar. Drugs 2020, 18(3), 146; https://doi.org/10.3390/md18030146
Received: 11 January 2020 / Revised: 23 February 2020 / Accepted: 24 February 2020 / Published: 29 February 2020
(This article belongs to the Special Issue Application of Marine Chitin and Chitosan)
A convenient and sensitive triple-wavelength overlapping resonance Rayleigh scattering (TWO-RRS) method for the detection of chito-oligosaccharides (COS) was proposed based on enhancing the rigid surface of porous reticular spatial structure of gelatin and COS by introducing allura red AC (AR). The interaction and resultant porous reticular spatial structure were characterized with transmission electron microscopy (TEM), RRS, and UV-Vis spectroscopy. The results indicated that gelatin and COS formed porous reticular spatial structure with an average diameter of 1.5–2.0 μm, and the RRS value of COS-AR-gelatin ternary system with gelatin participation was significantly higher than that of COS-AR binary system. Under the optimal conditions, the enhanced TWO-RRS intensity of the system was linearly proportional to COS concentration in the range of 0.30–2.50 μg/mL, and the regression equation was ΔI = 4933.2c − 446.21 with R2 = 0.9980. The limit of detection was 0.0478 μg/mL. So, a new method for the detection of COS was established and verified in the health products with satisfactory results. View Full-Text
Keywords: chito-oligosaccharides; allura red AC; gelatin; triple-wavelength overlapping resonance Rayleigh scattering chito-oligosaccharides; allura red AC; gelatin; triple-wavelength overlapping resonance Rayleigh scattering
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MDPI and ACS Style

Zou, W.; Sun, Z.; Su, Z.; Bai, Y. Application of Gelatin Decorated with Allura Red as Resonance Rayleigh Scattering Sensor to Detect Chito-Oligosaccharides. Mar. Drugs 2020, 18, 146.

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