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Open AccessArticle

Genome Sequencing and Analysis of Thraustochytriidae sp. SZU445 Provides Novel Insights into the Polyunsaturated Fatty Acid Biosynthesis Pathway

1
Guangdong Technology Research Center for Marine Algal Bioengineering, Guangdong Key Laboratory of Plant Epigenetics, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen 518060, China
2
Shenzhen Key Laboratory of Marine Biological Resources and Ecology Environment, Shenzhen Key Laboratory of Microbial Genetic Engineering, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen 518055, China
3
Longhua Innovation Institute for Biotechnology, Shenzhen University, Shenzhen 518060, China
4
School of Biomedical Sciences, The Chinese University of Hong Kong, Hong Kong 999077, China
5
Guangdong Research Center on Reproductive Control and Breeding Technology of Indigenous Valuable Fish Species, Fisheries College, Guangdong Ocean University, Zhanjiang 524088, China
*
Author to whom correspondence should be addressed.
Co-first author.
Mar. Drugs 2020, 18(2), 118; https://doi.org/10.3390/md18020118
Received: 23 December 2019 / Revised: 6 February 2020 / Accepted: 14 February 2020 / Published: 18 February 2020
Thraustochytriidae sp. have broadly gained attention as a prospective resource for the production of omega-3 fatty acids production in significant quantities. In this study, the whole genome of Thraustochytriidae sp. SZU445, which produces high levels of docosapentaenoic acid (DPA) and docosahexaenoic acid (DHA), was sequenced and subjected to protein annotation. The obtained clean reads (63.55 Mb in total) were assembled into 54 contigs and 25 scaffolds, with maximum and minimum lengths of 400 and 0.0054 Mb, respectively. A total of 3513 genes (24.84%) were identified, which could be classified into six pathways and 44 pathway groups, of which 68 genes (1.93%) were involved in lipid metabolism. In the Gene Ontology database, 22,436 genes were annotated as cellular component (8579 genes, 38.24%), molecular function (5236 genes, 23.34%), and biological process (8621 genes, 38.42%). Four enzymes corresponding to the classic fatty acid synthase (FAS) pathway and three enzymes corresponding to the classic polyketide synthase (PKS) pathway were identified in Thraustochytriidae sp. SZU445. Although PKS pathway-associated dehydratase and isomerase enzymes were not detected in Thraustochytriidae sp. SZU445, a putative DHA- and DPA-specific fatty acid pathway was identified. View Full-Text
Keywords: whole-genome sequencing; docosahexaenoic acid (DHA); polyunsaturated fatty acid; fatty acid synthesis pathway; polyketide synthase pathway whole-genome sequencing; docosahexaenoic acid (DHA); polyunsaturated fatty acid; fatty acid synthesis pathway; polyketide synthase pathway
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Zhu, X.; Li, S.; Liu, L.; Li, S.; Luo, Y.; Lv, C.; Wang, B.; Cheng, C.H.K.; Chen, H.; Yang, X. Genome Sequencing and Analysis of Thraustochytriidae sp. SZU445 Provides Novel Insights into the Polyunsaturated Fatty Acid Biosynthesis Pathway. Mar. Drugs 2020, 18, 118.

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