Serine Protease from Nereis virens Inhibits H1299 Lung Cancer Cell Proliferation via the PI3K/AKT/mTOR Pathway
Abstract
:1. Introduction
2. Results and Discussion
2.1. NAP Inhibits the Growth and Migration of H1299 Cells
2.2. NAP-Induced G0/G1 Phase Block in H1299 Cells
2.3. Influence of NAP on the Transcriptome of H1299 Cells
2.4. Influence of NAP on the ERK/MAPK Pathway in H1299 Cells
2.5. Influence of NAP on the PI3K/AKT/mTOR Pathway in H1299 Cells
2.6. NAP-Induced H1299 Cell Apoptosis Involves the PI3K/AKT/mTOR and ERK/MAPK Pathways
3. Materials and Methods
3.1. Cell Culture
3.2. Material Sources
3.3. Clonogenic Survival Assay
3.4. Cell Migration (Scratch Wound) Assay
3.5. Cell Cycle Experiment
3.6. RNA Extraction and Integrity Test
3.7. Western Blotting
3.8. Statistical Analysis
4. Conclusions
Author Contributions
Funding
Conflicts of Interest
References
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Chen, Y.; Tang, Y.; Tang, Y.; Yang, Z.; Ding, G. Serine Protease from Nereis virens Inhibits H1299 Lung Cancer Cell Proliferation via the PI3K/AKT/mTOR Pathway. Mar. Drugs 2019, 17, 366. https://doi.org/10.3390/md17060366
Chen Y, Tang Y, Tang Y, Yang Z, Ding G. Serine Protease from Nereis virens Inhibits H1299 Lung Cancer Cell Proliferation via the PI3K/AKT/mTOR Pathway. Marine Drugs. 2019; 17(6):366. https://doi.org/10.3390/md17060366
Chicago/Turabian StyleChen, Yanan, Yunping Tang, Yanhua Tang, Zuisu Yang, and Guofang Ding. 2019. "Serine Protease from Nereis virens Inhibits H1299 Lung Cancer Cell Proliferation via the PI3K/AKT/mTOR Pathway" Marine Drugs 17, no. 6: 366. https://doi.org/10.3390/md17060366