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Open AccessArticle

A Comparative Study on Asymmetric Reduction of Ketones Using the Growing and Resting Cells of Marine-Derived Fungi

1
School of Marine Sciences, Sun Yat-Sen University, Guangzhou 510275, China
2
Guangdong Provincial Key Laboratory of Marine Resources and Coastal Engineering, Guangzhou 510275, China
3
South China Sea Bio-Resource Exploitation and Utilization Collaborative Innovation Center, Sun Yat-Sen University, Guangzhou 510275, China
4
Departamento de Química, Faculdade de Ciências, UNESP, Bauru 17033-360, Brazil
*
Author to whom correspondence should be addressed.
Mar. Drugs 2018, 16(2), 62; https://doi.org/10.3390/md16020062
Received: 16 January 2018 / Revised: 1 February 2018 / Accepted: 3 February 2018 / Published: 14 February 2018
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Abstract

Whole-cell biocatalysts offer a highly enantioselective, minimally polluting route to optically active alcohols. Currently, most of the whole-cell catalytic performance involves resting cells rather than growing cell biotransformation, which is one-step process that benefits from the simultaneous growth and biotransformation, eliminating the need for catalysts preparation. In this paper, asymmetric reduction of 14 aromatic ketones to the corresponding enantiomerically pure alcohols was successfully conducted using the growing and resting cells of marine-derived fungi under optimized conditions. Good yields and excellent enantioselectivities were achieved with both methods. Although substrate inhibition might be a limiting factor for growing cell biotransformation, the selected strain can still completely convert 10-mM substrates into the desired products. The resting cell biotransformation showed a capacity to be recycled nine times without a significant decrease in the activity. This is the first study to perform asymmetric reduction of ketones by one-step growing cell biotransformation. View Full-Text
Keywords: growing cells; resting cells; asymmetric reduction; marine fungi; chiral alcohols growing cells; resting cells; asymmetric reduction; marine fungi; chiral alcohols
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Liu, H.; Chen, B.-S.; De Souza, F.Z.R.; Liu, L. A Comparative Study on Asymmetric Reduction of Ketones Using the Growing and Resting Cells of Marine-Derived Fungi. Mar. Drugs 2018, 16, 62.

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