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Open AccessArticle

Purification and Characterization of a Biofilm-Degradable Dextranase from a Marine Bacterium

by 1,2,3, 2,4, 1,3,5, 1,3,5, 1,3,5 and 1,3,5,*
1
Jiangsu Marine Resources Development Research Institute, Huaihai Institute of Technology, Lianyungang 222005, China
2
Key Laboratory of Marine Biology, Nanjing Agricultural University, Nanjing 210000, China
3
Co-Innovation Center of Jiangsu Marine Bio-industry Technology, Huaihai Institute of Technology, Lianyungang 222005, China
4
State Key Laboratory of Marine Environmental Science, College of Ocean and Earth Sciences, Xiamen University, Xiamen 361005, China
5
College of Marine Life and Fisheries, Huahai Institute of Technology, Lianyungang 222005, China
*
Author to whom correspondence should be addressed.
Mar. Drugs 2018, 16(2), 51; https://doi.org/10.3390/md16020051
Received: 18 November 2017 / Revised: 27 January 2018 / Accepted: 31 January 2018 / Published: 7 February 2018
This study evaluated the ability of a dextranase from a marine bacterium Catenovulum sp. (Cadex) to impede formation of Streptococcus mutans biofilms, a primary pathogen of dental caries, one of the most common human infectious diseases. Cadex was purified 29.6-fold and had a specific activity of 2309 U/mg protein and molecular weight of 75 kDa. Cadex showed maximum activity at pH 8.0 and 40 °C and was stable at temperatures under 30 °C and at pH ranging from 5.0 to 11.0. A metal ion and chemical dependency study showed that Mn2+ and Sr2+ exerted positive effects on Cadex, whereas Cu2+, Fe3+, Zn2+, Cd2+, Ni2+, and Co2+ functioned as inhibitors. Several teeth rinsing product reagents, including carboxybenzene, ethanol, sodium fluoride, and xylitol were found to have no effects on Cadex activity. A substrate specificity study showed that Cadex specifically cleaved the α-1,6 glycosidic bond. Thin layer chromatogram and high-performance liquid chromatography indicated that the main hydrolysis products were isomaltoogligosaccharides. Crystal violet staining and scanning electron microscopy showed that Cadex impeded the formation of S. mutans biofilm to some extent. In conclusion, Cadex from a marine bacterium was shown to be an alkaline and cold-adapted endo-type dextranase suitable for development of a novel marine agent for the treatment of dental caries. View Full-Text
Keywords: marine agent; Catenovulum; alkaline and cold-adapted dextranase; isomaltoogligosaccharides; biofilm; dental caries marine agent; Catenovulum; alkaline and cold-adapted dextranase; isomaltoogligosaccharides; biofilm; dental caries
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MDPI and ACS Style

Ren, W.; Cai, R.; Yan, W.; Lyu, M.; Fang, Y.; Wang, S. Purification and Characterization of a Biofilm-Degradable Dextranase from a Marine Bacterium. Mar. Drugs 2018, 16, 51.

AMA Style

Ren W, Cai R, Yan W, Lyu M, Fang Y, Wang S. Purification and Characterization of a Biofilm-Degradable Dextranase from a Marine Bacterium. Marine Drugs. 2018; 16(2):51.

Chicago/Turabian Style

Ren, Wei; Cai, Ruanhong; Yan, Wanli; Lyu, Mingsheng; Fang, Yaowei; Wang, Shujun. 2018. "Purification and Characterization of a Biofilm-Degradable Dextranase from a Marine Bacterium" Mar. Drugs 16, no. 2: 51.

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