Next Article in Journal
Deep Sea Water Modulates Blood Pressure and Exhibits Hypolipidemic Effects via the AMPK-ACC Pathway: An in Vivo Study
Previous Article in Journal
Cembrane Derivatives from the Soft Corals, Sinularia gaweli and Sinularia flexibilis
Open AccessArticle

Synchronized Regulation of Different Zwitterionic Metabolites in the Osmoadaption of Phytoplankton

Institute of Inorganic and Analytical Chemistry, Friedrich Schiller University, Lessingstr. 8, D-07743 Jena, Germany
*
Author to whom correspondence should be addressed.
Mar. Drugs 2013, 11(6), 2168-2182; https://doi.org/10.3390/md11062168
Received: 8 April 2013 / Revised: 23 April 2013 / Accepted: 28 May 2013 / Published: 17 June 2013
The ability to adapt to different seawater salinities is essential for cosmopolitan marine phytoplankton living in very diverse habitats. In this study, we examined the role of small zwitterionic metabolites in the osmoadaption of two common microalgae species Emiliania huxleyi and Prorocentrum minimum. By cultivation of the algae under salinities between 16‰ and 38‰ and subsequent analysis of dimethylsulfoniopropionate (DMSP), glycine betaine (GBT), gonyol, homarine, trigonelline, dimethylsulfonioacetate, trimethylammonium propionate, and trimethylammonium butyrate using HPLC-MS, we could reveal two fundamentally different osmoadaption mechanisms. While E. huxleyi responded with cell size reduction and a nearly constant ratio between the major metabolites DMSP, GBT and homarine to increasing salinity, osmolyte composition of P. minimum changed dramatically. In this alga DMSP concentration remained nearly constant at 18.6 mM between 20‰ and 32‰ but the amount of GBT and dimethylsulfonioacetate increased from 4% to 30% of total investigated osmolytes. Direct quantification of zwitterionic metabolites via LC-MS is a powerful tool to unravel the complex osmoadaption and regulation mechanisms of marine phytoplankton. View Full-Text
Keywords: Emiliania huxleyi; Prorocentrum minimum; dinoflagellates; haptophytes dimethylsulfoniopropionate (DMSP); gonyol; dimethylsulfide (DMS); osmoadaption Emiliania huxleyi; Prorocentrum minimum; dinoflagellates; haptophytes dimethylsulfoniopropionate (DMSP); gonyol; dimethylsulfide (DMS); osmoadaption
Show Figures

Figure 1

MDPI and ACS Style

Gebser, B.; Pohnert, G. Synchronized Regulation of Different Zwitterionic Metabolites in the Osmoadaption of Phytoplankton. Mar. Drugs 2013, 11, 2168-2182.

Show more citation formats Show less citations formats

Article Access Map by Country/Region

1
Only visits after 24 November 2015 are recorded.
Search more from Scilit
 
Search
Back to TopTop