Identification of Three Genes Encoding for the Late Acyltransferases of Lipid A in Cronobacter sakazakii
1
State Key Laboratory of Food Science and Technology, Jiangnan University, 1800 Lihu Road, Wuxi 214122, China
2
Key Laboratory of Industrial Biotechnology of Ministry of Education, Jiangnan University, 1800 Lihu Road, Wuxi 214122, China
*
Author to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Mar. Drugs 2013, 11(2), 377-386; https://doi.org/10.3390/md11020377
Received: 10 December 2012 / Revised: 6 January 2013 / Accepted: 9 January 2013 / Published: 31 January 2013
Lipid A, the hydrophobic anchor of lipopolysaccharide, is an essential component in the outer membrane of most Gram-negative bacteria. Food-borne pathogen Cronobacter sakazakii synthesizes two lipid A species, differing by the length of the secondary acyl chain. In this work, we identified three genes ESA02293, ESA02951 and ESA01386 encoding for the late acyltransferases of lipid A biosynthesis pathway in C. sakazakii. Based on the sequence alignment, proteins YP_001438378.1 encoded by ESA02293, YP_001439016.1 encoded by ESA02951, and YP_001437482.1 encoded by ESA01386 are homologous to E. coli LpxL, LpxP and LpxM, respectively. Functions of the three acyltransferases were confirmed by overexpressing the genes in E. coli, isolating lipid As and analyzing their structures using an ESI/MS. C. sakazakii LpxL and LpxM transfer a C14:0 secondary acyl chain to the 2′- and 3′-position of lipid A, respectively. C. sakazakii LpxP can transfer either a C16:1 or a C14:0 secondary acyl chains to the 2′-position of lipid A.
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Keywords:
Cronobacter sakazakii; lipid A; acyltransferase; lpxL; lpxM; lpxP
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MDPI and ACS Style
Cai, L.; Li, Y.; Tao, G.; Guo, W.; Zhang, C.; Wang, X. Identification of Three Genes Encoding for the Late Acyltransferases of Lipid A in Cronobacter sakazakii. Mar. Drugs 2013, 11, 377-386.
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