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Volume 40
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10.21775/cimb.040.165
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Current Issues in Molecular Biology is published by MDPI from Volume 43 Issue 1 (2021). Previous articles were published by another publisher in Open Access under a CC-BY (or CC-BY-NC-ND) licence, and they are hosted by MDPI on mdpi.com as a courtesy and upon agreement with Caister Press.
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Review

Crystallographic Structure Determination of Bacteriophage Endolysins

by
Marta Sanz-Gaitero
1,2,3 and
Mark J. van Raaij
1,*
1
Centro Nacional de Biotecnologia, CSIC, Calle Darwin 3, E-28049 Madrid, Spain
2
Cork Institute of Technology, Bishopstown, Cork T12 P928, Ireland
3
Current address: Centre for Molecular Medicine Norway, University of Oslo, Gaustadalléen 21, 0349 Oslo, Norway
*
Author to whom correspondence should be addressed.
Curr. Issues Mol. Biol. 2021, 40(1), 165-188; https://doi.org/10.21775/cimb.040.165
Submission received: 5 March 2020 / Revised: 10 April 2020 / Accepted: 12 May 2020 / Published: 23 June 2020
Versions Notes

Abstract

Bacteriophages produce endolysins that target and cleave the hosts peptidoglycan to release their progeny at the end of the infection cycle. These proteins can be used for the eradication of pathogenic bacteria, but also for their detection. Endolysins may contain a single catalytic domain or several domains, including a cell wall binding domain. To understand their function in detail and design mutated or chimeric molecules with novel properties, knowledge of their structures and detailed mechanisms is necessary. X-ray protein crystallography is an excellent method to obtain high-resolution structures of biological macromolecules, and here we describe the method and the folds of known endolysin domains.
Keywords: crystallographic; structure; determination; bacteriophage; endolysins crystallographic; structure; determination; bacteriophage; endolysins

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MDPI and ACS Style

Sanz-Gaitero, M.; van Raaij, M.J. Crystallographic Structure Determination of Bacteriophage Endolysins. Curr. Issues Mol. Biol. 2021, 40, 165-188. https://doi.org/10.21775/cimb.040.165

AMA Style

Sanz-Gaitero M, van Raaij MJ. Crystallographic Structure Determination of Bacteriophage Endolysins. Current Issues in Molecular Biology. 2021; 40(1):165-188. https://doi.org/10.21775/cimb.040.165

Chicago/Turabian Style

Sanz-Gaitero, Marta, and Mark J. van Raaij. 2021. "Crystallographic Structure Determination of Bacteriophage Endolysins" Current Issues in Molecular Biology 40, no. 1: 165-188. https://doi.org/10.21775/cimb.040.165

APA Style

Sanz-Gaitero, M., & van Raaij, M. J. (2021). Crystallographic Structure Determination of Bacteriophage Endolysins. Current Issues in Molecular Biology, 40(1), 165-188. https://doi.org/10.21775/cimb.040.165

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