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Current Issues in Molecular Biology is published by MDPI from Volume 43 Issue 1 (2021). Previous articles were published by another publisher in Open Access under a CC-BY (or CC-BY-NC-ND) licence, and they are hosted by MDPI on mdpi.com as a courtesy and upon agreement with Caister Press.
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Review

Analysis of Specific Bacteria from Environmental Samples Using a Quantitative Polymerase Chain Reaction

by
Clifford F. Brunk
1,2,*,
Jinliang Li
2 and
Erik Avaniss-Aghajani
2
1
Biology Department, University of California, 621 Charles E. Young Dr. So, Los Angeles, CA 90095-1606, USA
2
Laragen Inc., 10755 Venice Ave., Los Angeles, CA 90034, USA
*
Author to whom correspondence should be addressed.
Curr. Issues Mol. Biol. 2002, 4(1), 13-18; https://doi.org/10.21775/cimb.004.013
Submission received: 10 September 2001 / Revised: 10 October 2001 / Accepted: 11 December 2001 / Published: 13 February 2002
Versions Notes

Abstract

This article describes the use of quantitative PCR for measuring bacterial abundance in environmental samples. The two approaches discussed are: (1) The use of an internal PCR standard constructed to be the same size and have the same sequence as the primary amplification target, but differing from the primary target by 2–3 bases, corresponding to a unique restriction site. This allows the amount of target amplicon to be compared with the internal standard and circumvents the problem of differential amplification efficiencies when using dissimilar targets and standard amplicons. (2) The use of Taqman technology (Applied Biosystems, Foster City, California) with a dual labeled oligonucleotide probe which binds internal to the PCR primers. The detection of Bacteroides is used as an example for both approaches.

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MDPI and ACS Style

Brunk, C.F.; Li, J.; Avaniss-Aghajani, E. Analysis of Specific Bacteria from Environmental Samples Using a Quantitative Polymerase Chain Reaction. Curr. Issues Mol. Biol. 2002, 4, 13-18. https://doi.org/10.21775/cimb.004.013

AMA Style

Brunk CF, Li J, Avaniss-Aghajani E. Analysis of Specific Bacteria from Environmental Samples Using a Quantitative Polymerase Chain Reaction. Current Issues in Molecular Biology. 2002; 4(1):13-18. https://doi.org/10.21775/cimb.004.013

Chicago/Turabian Style

Brunk, Clifford F., Jinliang Li, and Erik Avaniss-Aghajani. 2002. "Analysis of Specific Bacteria from Environmental Samples Using a Quantitative Polymerase Chain Reaction" Current Issues in Molecular Biology 4, no. 1: 13-18. https://doi.org/10.21775/cimb.004.013

APA Style

Brunk, C. F., Li, J., & Avaniss-Aghajani, E. (2002). Analysis of Specific Bacteria from Environmental Samples Using a Quantitative Polymerase Chain Reaction. Current Issues in Molecular Biology, 4(1), 13-18. https://doi.org/10.21775/cimb.004.013

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