Diterpenoids from Plectranthus spp. as Potential Chemotherapeutic Agents via Apoptosis
Abstract
:1. Introduction
2. Results
2.1. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl Tetrazolium Bromide (MTT) Assay
2.2. Reactive Oxygen Species (ROS)
2.3. Mitochondrial Membrane Potential
2.4. Mitochondrial Copy Number
2.5. Mitochondrial DNA Damage
2.6. Nuclear DNA Damage
2.7. Gene Expression
3. Discussion
4. Materials and Methods
4.1. Plant Material
4.2. Isolation of Abietane Diterpenes
4.3. Cells and Culture Conditions
4.4. MTT Cell Viability Assay
4.5. Measurement of Intracellular Reactive Oxygen Species (ROS) Level
4.6. Mitochondrial Membrane Potential (MMP)
4.7. Isolation of Total Genomic DNA from Cell Lines
4.8. Mitochondrial Copy Number
4.9. Semi-Long-Run qRT-PCR (SLR-qRT-PCR)—Mitochondrial and Nuclear DNA Damage
4.10. Gene Expression
4.11. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Conflicts of Interest
Abbreviations
Deroy | 6,7-dehydroroyleanone |
Diroy | 7β,6β-dihydroxyroyleanone |
Parv | parvifloron D |
Roy | 7α-acetoxy-6β-hydroxyroyleanon |
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Śliwiński, T.; Sitarek, P.; Skała, E.; M. S. Isca, V.; Synowiec, E.; Kowalczyk, T.; Bijak, M.; Rijo, P. Diterpenoids from Plectranthus spp. as Potential Chemotherapeutic Agents via Apoptosis. Pharmaceuticals 2020, 13, 123. https://doi.org/10.3390/ph13060123
Śliwiński T, Sitarek P, Skała E, M. S. Isca V, Synowiec E, Kowalczyk T, Bijak M, Rijo P. Diterpenoids from Plectranthus spp. as Potential Chemotherapeutic Agents via Apoptosis. Pharmaceuticals. 2020; 13(6):123. https://doi.org/10.3390/ph13060123
Chicago/Turabian StyleŚliwiński, Tomasz, Przemysław Sitarek, Ewa Skała, Vera M. S. Isca, Ewelina Synowiec, Tomasz Kowalczyk, Michał Bijak, and Patrícia Rijo. 2020. "Diterpenoids from Plectranthus spp. as Potential Chemotherapeutic Agents via Apoptosis" Pharmaceuticals 13, no. 6: 123. https://doi.org/10.3390/ph13060123
APA StyleŚliwiński, T., Sitarek, P., Skała, E., M. S. Isca, V., Synowiec, E., Kowalczyk, T., Bijak, M., & Rijo, P. (2020). Diterpenoids from Plectranthus spp. as Potential Chemotherapeutic Agents via Apoptosis. Pharmaceuticals, 13(6), 123. https://doi.org/10.3390/ph13060123