Design of Smartphone-Assisted Point-of-Care Platform for Colorimetric Sensing of Uric Acid via Visible Light-Induced Oxidase-Like Activity of Covalent Organic Framework
Abstract
:1. Introduction
2. Materials and Methods
2.1. Chemicals and Reagents
2.2. Synthesis of TpBpy COF
2.3. Characterization of TpBpy COF
2.4. Steady-State Kinetic Studies of TpBpy COF as Oxidase-Mimic
2.5. Colorimetric Detection of UA
2.6. Detection of UA by the Smartphone Mode
2.7. Determination of UA Content in Real Samples
3. Results and Discussion
3.1. Synthesis and Characterizations of TpBpy COF
3.2. Oxidase-Like Activity of TpBpy COF under Visible Light Illumination
3.3. Analytical Performance for Colorimetric UA Sensing
3.4. Smartphone Sensing Platform for UA Detection
3.5. Determination of UA Content in Human Serum and Urine Samples
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Method | Sensitivity | Selectivity | Rapidity | Precision | Complex Sample Preparation Process | Expensive Instruments | Low Cost |
---|---|---|---|---|---|---|---|
Colorimetry | √ | √ | √ | х | х | х | √ |
HPLC | √ | √ | √ | √ | √ | √ | х |
Fluorescence | √ | √ | √ | √ | х | √ | √ |
Chemiluminescence | √ | х | √ | √ | √ | х | √ |
Electrochemistry | √ | √ | √ | √ | √ | х | √ |
Catalyst | Km (mM) | Vm (10−6 M min−1) | Ref. |
---|---|---|---|
TpBpy COF | 0.0948 | 4.31 × 10−6 | This work |
PtNPs@ZIF | 0.15 | 7.26 × 10−6 | [18] |
CuSNPs | 0.216 | 1.45 × 10−5 | [34] |
Fe3O4@MIL-100 (Fe) | 0.112 | 6.85 × 10−6 | [35] |
HRP | 0.4340 | 6.00 × 10−6 | [23] |
Material | Linear Range (μM) | LOD (μM) | Ref. |
---|---|---|---|
TpBpy COF | 5–80 | 1.7 | This work |
Uricase/graphitic carbon nitride | 10–100 | 8.9 | [36] |
Ni glancing angle deposition film | 0–6 | 3.3 | [37] |
Lanthanide-doped upconversion nanoparticles | 10–1000 | 2.86 | [38] |
Au/Ag nanoclusters | 5–50 | 5.1 | [39] |
TCPO-H2O2-rubrene | 10–1000 | 5 | [40] |
Photochemically reduced graphene oxide | 40–415 | 8 | [41] |
Sample | Added (μM) | Colorimetric Determination (μM) | Recovery (%) | RSD (%, n = 3) | Smartphone Determination (μM) | Recovery (%) | RSD (%, n = 3) |
---|---|---|---|---|---|---|---|
Urine | 0 | 24.3 ± 1.2 | 4.8 | 23.3 ± 0.8 | 3.3 | ||
10.0 | 34.7 ± 2.0 | 103.6 ± 2.0 | 5.6 | 34.1 ± 0.6 | 107.1 ± 5.6 | 1.6 | |
30.0 | 55.0 ± 1.1 | 102.3 ± 3.5 | 1.9 | 55.7 ± 0.6 | 107.8 ± 2.0 | 1.1 | |
50.0 | 73.0 ± 0.7 | 97.2 ± 1.3 | 0.9 | 73.8 ± 1.6 | 100.8 ± 3.2 | 2.3 | |
Serum | 0 | 4.5 ± 0.2 | 3.6 | 4.6 ± 0.1 | 2.4 | ||
10.0 | 14.3 ± 0.4 | 98.8 ± 3.5 | 2.7 | 14.3 ± 0.9 | 97.1 ± 0.8 | 5.9 | |
30.0 | 33.9 ± 0.4 | 98.1 ± 1.4 | 1.3 | 33.6 ± 0.4 | 96.6 ± 1.4 | 1.2 | |
50.0 | 54.5 ± 0.4 | 100.1 ± 0.9 | 0.7 | 54.7 ± 0.8 | 100.2 ± 1.6 | 1.4 |
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Kang, Q.; Xu, Y.; Chen, X. Design of Smartphone-Assisted Point-of-Care Platform for Colorimetric Sensing of Uric Acid via Visible Light-Induced Oxidase-Like Activity of Covalent Organic Framework. Sensors 2023, 23, 3881. https://doi.org/10.3390/s23083881
Kang Q, Xu Y, Chen X. Design of Smartphone-Assisted Point-of-Care Platform for Colorimetric Sensing of Uric Acid via Visible Light-Induced Oxidase-Like Activity of Covalent Organic Framework. Sensors. 2023; 23(8):3881. https://doi.org/10.3390/s23083881
Chicago/Turabian StyleKang, Qi, Yulong Xu, and Xuwei Chen. 2023. "Design of Smartphone-Assisted Point-of-Care Platform for Colorimetric Sensing of Uric Acid via Visible Light-Induced Oxidase-Like Activity of Covalent Organic Framework" Sensors 23, no. 8: 3881. https://doi.org/10.3390/s23083881