Magnetoelastic Monitoring System for Tracking Growth of Human Mesenchymal Stromal Cells
Abstract
:1. Introduction
2. Materials and Methods
2.1. Sensor Fabrication
2.2. Sensor Stage Fabrication
2.3. Detection System
2.4. hMSC Cell Culture
2.5. hMSC Cell Seeding
2.6. Sensitivity Curve
2.7. Longitudinal Tracking of Cell Loading
2.8. Staining, Imaging, and Counting of Cells on Sensors
3. Results and Discussion
3.1. Heterogeneity of Cell Distribution on Sensors
3.2. Evaluation of Sensor Sensitivity
3.3. Longitudinal Tracking of Cell Loading
3.4. Gelatin Surface Treatment
3.5. Effects of Sensor Activity on Cell Morphology
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Skinner, W.S.; Zhang, S.; Garcia, J.R.; Guldberg, R.E.; Ong, K.G. Magnetoelastic Monitoring System for Tracking Growth of Human Mesenchymal Stromal Cells. Sensors 2023, 23, 1832. https://doi.org/10.3390/s23041832
Skinner WS, Zhang S, Garcia JR, Guldberg RE, Ong KG. Magnetoelastic Monitoring System for Tracking Growth of Human Mesenchymal Stromal Cells. Sensors. 2023; 23(4):1832. https://doi.org/10.3390/s23041832
Chicago/Turabian StyleSkinner, William S., Sunny Zhang, Jasmine R. Garcia, Robert E. Guldberg, and Keat Ghee Ong. 2023. "Magnetoelastic Monitoring System for Tracking Growth of Human Mesenchymal Stromal Cells" Sensors 23, no. 4: 1832. https://doi.org/10.3390/s23041832