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Open AccessArticle

Anti-p53 Autoantibody Detection in Automatic Glass Capillary Immunoassay Platform for Screening of Oral Cavity Squamous Cell Carcinoma

by Yen-Heng Lin 1,2,*,†, Chih-Ching Wu 2,3,4,†, Wan-Ling Chen 1 and Kai-Ping Chang 2,4
1
Graduate Institute of Biomedical Engineering, Chang Gung University, Taoyuan 333, Taiwan
2
Department of Otolaryngology-Head & Neck Surgery, Chang Gung Memorial Hospital, Linkou, Taoyuan 333, Taiwan
3
Departments of Medical Biotechnology and Laboratory Science, Chang Gung University, Taoyuan 333, Taiwan
4
Molecular Medicine Research Center, Chang Gung University, Taoyuan 333, Taiwan
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Sensors 2020, 20(4), 971; https://doi.org/10.3390/s20040971
Received: 3 December 2019 / Revised: 7 February 2020 / Accepted: 8 February 2020 / Published: 11 February 2020
(This article belongs to the Special Issue Biosensors for Cancer Detection and Monitoring)
The incidence of oral squamous cell carcinoma (OSCC), which is one of the most common cancers worldwide, has been increasing. Serum anti-p53 autoantibody is one of the most sensitive biomarkers for OSCC. Currently, the most commonly used method on clinical screening platforms is the enzyme-linked immunosorbent assay, owing to its high specificity and repeatability. However, conducting immunoassays on 96-well plates is typically time consuming, thereby limiting its clinical applications for fast diagnosis and immediate prognosis of rapidly progressive diseases. The present study performed immunoassays in glass capillaries of 1-mm internal diameter, which increases the surface to volume ratio of the reaction, to shorten the time needed for immunoassay. The immunoassay was automated while using linear motorized stages and a syringe pump. The results indicated that, when compared with the 96-well plate immunoassay, the glass capillary immunoassay decreased the reaction time from typical 120 min to 45 min, reduced the amount of reagent from typical 50 µL to 15 µL, and required only simple equipment setup. Moreover, the limit of detection for glass capillary anti-p53 autoantibody immunoassay was 0.46 ng mL−1, which is close to the 0.19 ng mL−1 value of the conventional 96-well plate assay, and the glass capillary method had a broader detection range. The apparatus was used to detect the serum anti-p53 autoantibody concentration in clinical patients and compare its results with the conventional 96-well plate method results, which suggested that both of the methods detect the same trend in the relative concentration of serum anti-p53 autoantibody in healthy individuals or patients with OSCC. View Full-Text
Keywords: anti-p53 autoantibody; oral squamous cell carcinoma; enzyme-linked immunosorbent assay; microfluidics anti-p53 autoantibody; oral squamous cell carcinoma; enzyme-linked immunosorbent assay; microfluidics
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Lin, Y.-H.; Wu, C.-C.; Chen, W.-L.; Chang, K.-P. Anti-p53 Autoantibody Detection in Automatic Glass Capillary Immunoassay Platform for Screening of Oral Cavity Squamous Cell Carcinoma. Sensors 2020, 20, 971.

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