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Open AccessArticle

Multi-Electrode Array with a Planar Surface for Cell Patterning by Microprinting

1
Central European Institute of Technology, Brno University of Technology, Purkyňova 123, 616 00 Brno, Czech Republic
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Department of Biomedical Engineering, Faculty of Electrical Engineering and Communication, Brno University of Technology, Technická 12, 612 00 Brno, Czech Republic
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Department of Physiology, Faculty of Medicine, Masaryk University, Kamenice 5, 625 00 Brno, Czech Republic
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Department of Microelectronics, Faculty of Electrical Engineering and Communication, Brno University of Technology, Technická3058/10, 616 00 Brno, Czech Republic
*
Author to whom correspondence should be addressed.
Sensors 2019, 19(24), 5379; https://doi.org/10.3390/s19245379
Received: 8 October 2019 / Revised: 24 November 2019 / Accepted: 3 December 2019 / Published: 5 December 2019
(This article belongs to the Section Biomedical Sensors)
Multielectrode arrays (MEAs) are devices for non-invasive electrophysiological measurements of cell populations. This paper describes a novel fabrication method of MEAs with a fully planar surface. The surface of the insulation layer and the surface of the electrodes were on one plane; we named this device the planar MEA (pMEA). The main advantage of the pMEA is that it allows uniform contact between the pMEA surface and a substrate for positioning of microfluidic channels or microprinting of a cell adhesive layer. The fabrication of the pMEA is based on a low adhesive Au sacrificial peel-off layer. In divergence from conventional MEAs with recessed electrodes, the electrodes of the pMEA lead across the sloped edge of the insulation layer. To make this, the profile of the edge of the insulation layer was measured and the impedance of the planar electrodes was characterized. The impedance of the pMEA was comparable with the impedance of conventional MEA electrodes. The pMEA was tested for patterning HL-1 cells with a combination of imprinting fibronectin and coating by antifouling poly (l-lysine)-graft-poly(ethylene glycol) (PLL-g-PEG). The HL-1 cells remained patterned even at full confluency and presented spontaneous and synchronous beating activity. View Full-Text
Keywords: multielectrode array; microelectrode array; sacrificial layer; peel-off; HL-1 cells; microprinting; antifouling agent multielectrode array; microelectrode array; sacrificial layer; peel-off; HL-1 cells; microprinting; antifouling agent
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MDPI and ACS Style

Slavík, J.; Skopalík, J.; Provazník, I.; Hubálek, J. Multi-Electrode Array with a Planar Surface for Cell Patterning by Microprinting. Sensors 2019, 19, 5379.

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