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Sensors 2019, 19(2), 238; https://doi.org/10.3390/s19020238

Dual-Modal In Vivo Fluorescence/Photoacoustic Microscopy Imaging of Inflammation Induced by GFP-Expressing Bacteria

Cancer Center, Faculty of Health Sciences, University of Macau, Macau SAR 999078, China
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Received: 20 November 2018 / Revised: 10 December 2018 / Accepted: 10 December 2018 / Published: 10 January 2019
(This article belongs to the Special Issue Ultrasound Transducers)
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Abstract

In this study, dual-modal fluorescence and photoacoustic microscopy was performed for noninvasive and functional in vivo imaging of inflammation induced by green fluorescent protein (GFP) transfected bacteria in mice ear. Our imaging results demonstrated that the multimodal imaging technique is able to monitor the tissue immunovascular responses to infections with molecular specificity. Our study also indicated that the combination of photoacoustic and fluorescence microscopy imaging can simultaneously track the biochemical changes including the bacterial distribution and morphological change of blood vessels in the biological tissues with high resolution and enhanced sensitivity. Consequently, the developed method paves a new avenue for improving the understanding of the pathology mechanism of inflammation. View Full-Text
Keywords: GFP-expressing bacteria; biosensor; inflammation/infection; fluorescence imaging; photoacoustic microscopy GFP-expressing bacteria; biosensor; inflammation/infection; fluorescence imaging; photoacoustic microscopy
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
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Liu, Y.; Fu, L.; Xu, M.; Zheng, J.; Yuan, Z. Dual-Modal In Vivo Fluorescence/Photoacoustic Microscopy Imaging of Inflammation Induced by GFP-Expressing Bacteria. Sensors 2019, 19, 238.

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