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Adaptable Xerogel-Layered Amperometric Biosensor Platforms on Wire Electrodes for Clinically Relevant Measurements

Department of Chemistry, Gottwald Science Center, University of Richmond, Richmond, VA 23173, USA
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Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Sensors 2019, 19(11), 2584; https://doi.org/10.3390/s19112584
Received: 10 May 2019 / Revised: 3 June 2019 / Accepted: 4 June 2019 / Published: 6 June 2019
(This article belongs to the Special Issue Bio- and Chemical Sensors for Biomedical Applications)
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Abstract

Biosensing strategies that employ readily adaptable materials for different analytes, can be miniaturized into needle electrode form, and function in bodily fluids represent a significant step toward the development of clinically relevant in vitro and in vivo sensors. In this work, a general scheme for 1st generation amperometric biosensors involving layer-by-layer electrode modification with enzyme-doped xerogels, electrochemically-deposited polymer, and polyurethane semi-permeable membranes is shown to achieve these goals. With minor modifications to these materials, sensors representing potential point-of-care medical tools are demonstrated to be sensitive and selective for a number of conditions. The potential for bedside measurements or continuous monitoring of analytes may offer faster and more accurate clinical diagnoses for diseases such as diabetes (glucose), preeclampsia (uric acid), galactosemia (galactose), xanthinuria (xanthine), and sepsis (lactate). For the specific diagnostic application, the sensing schemes have been miniaturized to wire electrodes and/or demonstrated as functional in synthetic urine or blood serum. Signal enhancement through the incorporation of platinum nanoparticle film in the scheme offers additional design control within the sensing scheme. The presented sensing strategy has the potential to be applied to any disease that has a related biomolecule and corresponding oxidase enzyme and represents rare, adaptable, sensing capabilities. View Full-Text
Keywords: biosensors; biomedical; in vitro; in vivo; needle electrode; wire; sepsis; enzyme; electrochemical; platinum black; bodily fluids biosensors; biomedical; in vitro; in vivo; needle electrode; wire; sepsis; enzyme; electrochemical; platinum black; bodily fluids
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Hughes, L.B.; Labban, N.; Conway, G.E.; Pollock, J.A.; Leopold, M.C. Adaptable Xerogel-Layered Amperometric Biosensor Platforms on Wire Electrodes for Clinically Relevant Measurements. Sensors 2019, 19, 2584.

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