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Open AccessArticle

Membrane-Based Microwave-Mediated Electrochemical Immunoassay for the In Vitro, Highly Sensitive Detection of Osteoporosis-Related Biomarkers

1
School of Integrative Engineering, Chung-Ang University, 84 Heukseok-Ro, Dongjak-Gu, Seoul 06974, Korea
2
Department of Applied Chemistry, Research Center for Biomicrosensing Technology, Kyushu Institute of Technology, Fukuoka 804-8550, Japan
*
Author to whom correspondence should be addressed.
Sensors 2018, 18(9), 2933; https://doi.org/10.3390/s18092933
Received: 20 July 2018 / Revised: 19 August 2018 / Accepted: 31 August 2018 / Published: 4 September 2018
(This article belongs to the Special Issue Biosensors for the Detection of Biomarkers)
Highly sensitive and multiplexed in vitro detection of osteoporosis-related biochemical markers were carried out based on the membrane-based microwave-mediated electrochemical immunoassay (MMeEIA), where we can dramatically reduce the sample preparation time by shortening the incubation time of conjugation to obtain sensitive detection based on three dimensional conjugation of antibodies with target antigens in nylon membrane disk. C-terminal cross-linked telopeptide of type I collagen (CTx), Osteocalcin (OC), parathyroid hormone (PTH), and N-terminal propeptide of type I collagen (P1NP), which can be utilized to monitor the progress of osteoporosis, were quantified using their corresponding antibody immobilized in membranes. Coefficient of variations in this intra- and inter-assays were within 8.0% for all markers. When compared with data obtained from clinically used standard equipment (Roche modular E170), their coefficients of determination, R2 values, are mostly more than 0.9. They show that the results obtained from MMeEIA are in good agreement with that from the conventional clinical instruments. View Full-Text
Keywords: membrane-based microwave-mediated electrochemical immunoassay (MMeEIA); bone turnover marker; osteoporosis; C-terminal cross-linked telopeptide of type I collagen (CTx); osteocalcin (OC); parathyroid hormone (PTH); N-terminal propeptide of type I collagen (P1NP) membrane-based microwave-mediated electrochemical immunoassay (MMeEIA); bone turnover marker; osteoporosis; C-terminal cross-linked telopeptide of type I collagen (CTx); osteocalcin (OC); parathyroid hormone (PTH); N-terminal propeptide of type I collagen (P1NP)
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Kim, H.Y.; Sato, S.; Takenaka, S.; Lee, M.-H. Membrane-Based Microwave-Mediated Electrochemical Immunoassay for the In Vitro, Highly Sensitive Detection of Osteoporosis-Related Biomarkers. Sensors 2018, 18, 2933.

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