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Sensors 2018, 18(5), 1494; https://doi.org/10.3390/s18051494

Fast and Cost-Effective Biochemical Spectrophotometric Analysis of Solution of Insect “Blood” and Body Surface Elution

Faculty of Biology, Animal Sciences and Bioeconomy, Institute of Biological Basis of Animal Production, University of Life Sciences in Lublin, Akademicka 13, 20950 Lublin, Poland
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Received: 31 March 2018 / Revised: 5 May 2018 / Accepted: 8 May 2018 / Published: 9 May 2018
(This article belongs to the Section Biosensors)
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Abstract

Using insect hemolymph (“blood”) and insect body surface elutions, researchers can perform rapid and cheap biochemical analyses to determine the insect’s immunology status. The authors of this publication describe a detailed methodology for a quick marking of the concentration of total proteins and evaluation of the proteolytic system activity (acid, neutral, and alkaline proteases and protease inhibitors), as well as a methodology for quick “liver” tests in insects: alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), and urea and glucose concentration analyses. The meaning and examples of an interpretation of the results of the presented methodology for biochemical parameter determination are described for the example of honey bees. View Full-Text
Keywords: hemolymph; immunology; resistance; biochemical parameters; surface proteins; proteolytic system; proteases; protease inhibitors; absorbance; activity hemolymph; immunology; resistance; biochemical parameters; surface proteins; proteolytic system; proteases; protease inhibitors; absorbance; activity
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Łoś, A.; Strachecka, A. Fast and Cost-Effective Biochemical Spectrophotometric Analysis of Solution of Insect “Blood” and Body Surface Elution. Sensors 2018, 18, 1494.

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