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Open AccessArticle

Experimental Study of the Oriented Immobilization of Antibodies on Photonic Sensing Structures by Using Protein A as an Intermediate Layer

Nanophotonics Technology Center, Universitat Politècnica de València, Camino de Vera s/n, 46022 Valencia, Spain
Departamento de Química, Instituto Universitario de Investigación de Reconocimiento Molecular y Desarrollo Tecnológico (IDM), Universitat Politècnica de València, Universitat de València, 46022 Valencia, Spain
Author to whom correspondence should be addressed.
Sensors 2018, 18(4), 1012;
Received: 19 February 2018 / Revised: 20 March 2018 / Accepted: 27 March 2018 / Published: 28 March 2018
(This article belongs to the Special Issue Protein-Based Biosensors)
A proper antibody immobilization on a biosensor is a crucial step in order to obtain a high sensitivity to be able to detect low target analyte concentrations. In this paper, we present an experimental study of the immobilization process of antibodies as bioreceptors on a photonic ring resonator sensor. A protein A intermediate layer was created on the sensor surface in order to obtain an oriented immobilization of the antibodies, which enhances the interaction with the target antigens to be detected. The anti-bovine serum albumin (antiBSA)-bovine serum albumin (BSA) pair was used as a model for our study. An opto-fluidic setup was developed in order to flow the different reagents and, simultaneously, to monitor in real-time the spectral response of the photonic sensing structure. The antiBSA immobilization and the BSA detection, their repeatability, and specificity were studied in different conditions of the sensor surface. Finally, an experimental limit of detection for BSA recognition of only 1 ng/mL was obtained. View Full-Text
Keywords: Integrated photonics; ring resonator; sensor; biosensing Integrated photonics; ring resonator; sensor; biosensing
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MDPI and ACS Style

Caroselli, R.; García Castelló, J.; Escorihuela, J.; Bañuls, M.J.; Maquieira, Á.; García-Rupérez, J. Experimental Study of the Oriented Immobilization of Antibodies on Photonic Sensing Structures by Using Protein A as an Intermediate Layer. Sensors 2018, 18, 1012.

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