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Sensors 2018, 18(12), 4298; https://doi.org/10.3390/s18124298

Etched Fiber Bragg Grating Biosensor Functionalized with Aptamers for Detection of Thrombin

1
Laboratory of Biosensors and Bioinstruments, National Laboratory Astana, Nazarbayev University, Astana 010000, Kazakhstan
2
School of Engineering, Nazarbayev University, Astana 010000, Kazakhstan
*
Author to whom correspondence should be addressed.
Received: 24 October 2018 / Revised: 26 November 2018 / Accepted: 3 December 2018 / Published: 6 December 2018
(This article belongs to the Special Issue Optical Bio Sensing)
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Abstract

A biosensor based on an etched Fiber Bragg Grating (EFBG) for thrombin detection is reported. The sensing system is based on a Fiber Bragg Grating (FBG) with a Bragg wavelength of 1550 nm, wet-etched in hydrofluoric acid (HF) for ~27 min, to achieve sensitivity to a refractive index (RI) of 17.4 nm/RIU (refractive index unit). Subsequently, in order to perform a selective detection of thrombin, the EFBG has been functionalized with silane-coupling agent 3-(aminopropyl)triethoxysilane (APTES) and a cross-linker, glutaraldehyde, for the immobilization of thrombin-binding aptamer. The biosensor has been validated for thrombin detection in concentrations ranging from 10 nM to 80 nM. The proposed sensor presents advantages with respect to other sensor configurations, based on plasmonic resonant tilted FBG or Long Period Grating (LPG), for thrombin detection. Firstly, fabricating an EFBG only requires chemical etching. Moreover, the functionalization method used in this study (silanization) allows the avoidance of complicated and expensive fabrications, such as thin film sputtering or chemical vapor deposition. Due to their characteristics, EFBG sensors are easier to multiplex and can be used in vivo. This opens new possibilities for the detection of thrombin in clinical settings. View Full-Text
Keywords: Optical fiber biosensors; fiber Bragg gratings (FBG); refractive index sensor; aptamer; thrombin; biosensor Optical fiber biosensors; fiber Bragg gratings (FBG); refractive index sensor; aptamer; thrombin; biosensor
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
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Bekmurzayeva, A.; Dukenbayev, K.; Shaimerdenova, M.; Bekniyazov, I.; Ayupova, T.; Sypabekova, M.; Molardi, C.; Tosi, D. Etched Fiber Bragg Grating Biosensor Functionalized with Aptamers for Detection of Thrombin. Sensors 2018, 18, 4298.

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