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Sensors 2018, 18(1), 290; https://doi.org/10.3390/s18010290

Wide-Field Fluorescence Microscopy of Real-Time Bioconjugation Sensing

1
Faculty of Physics, Astronomy and Informatics, Nicolaus Copernicus University, Grudziadzka 5, 87-100 Torun, Poland
2
Baltic Institute of Technology, Al. Zwycięstwa 96/98, 81-451 Gdynia, Poland
3
Institute of Physical Chemistry Polish Academy of Sciences, ul. Kasprzaka 44/52, 01-224 Warsaw, Poland
*
Authors to whom correspondence should be addressed.
Received: 6 December 2017 / Revised: 12 January 2018 / Accepted: 16 January 2018 / Published: 19 January 2018
(This article belongs to the Special Issue Novel Approaches to Biosensing with Nanoparticles)
Full-Text   |   PDF [1485 KB, uploaded 19 January 2018]   |  

Abstract

We apply wide-field fluorescence microscopy to measure real-time attachment of photosynthetic proteins to plasmonically active silver nanowires. The observation of this effect is enabled, on the one hand, by sensitive detection of fluorescence and, on the other hand, by plasmonic enhancement of protein fluorescence. We examined two sample configurations with substrates being a bare glass coverslip and a coverslip functionalized with a monolayer of streptavidin. The different preparation of the substrate changes the observed behavior as far as attachment of the protein is concerned as well as its subsequent photobleaching. For the latter substrate the conjugation process is measurably slower. The described method can be universally applied in studying protein-nanostructure interactions for real-time fluorescence-based sensing. View Full-Text
Keywords: fluorescence imaging; bioconjugation; plasmon enhancement; silver nanowire fluorescence imaging; bioconjugation; plasmon enhancement; silver nanowire
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Szalkowski, M.; Sulowska, K.; Grzelak, J.; Niedziółka-Jönsson, J.; Roźniecka, E.; Kowalska, D.; Maćkowski, S. Wide-Field Fluorescence Microscopy of Real-Time Bioconjugation Sensing. Sensors 2018, 18, 290.

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