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Sensors 2017, 17(11), 2684;

A Novel Detection Method of Human Serum Albumin Based on the Poly(Thymine)-Templated Copper Nanoparticles

School of Life Sciences, Central South University, Changsha 410013, China
These authors contributed equally to this work.
Author to whom correspondence should be addressed.
Received: 12 October 2017 / Revised: 13 November 2017 / Accepted: 14 November 2017 / Published: 21 November 2017
(This article belongs to the Special Issue Biosensors for Cancer Biomarkers)
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In this work, we developed a facile fluorescence method for quantitative detection of human serum albumin (HSA) based on the inhibition of poly(thymine) (poly T)-templated copper nanoparticles (CuNPs) in the presence of HSA. Under normal circumstances, poly T-templated CuNPs can display strong fluorescence with excitation/emission peaks at 340/610 nm. However, in the presence of HSA, it will absorb cupric ion, which will prevent the formation of CuNPs. As a result, the fluorescence intensity will become obviously lower in the presence of HSA. The analyte HSA concentration had a proportional linear relationship with the fluorescence intensity of CuNPs. The detection limit for HSA was 8.2 × 10−8 mol·L−1. Furthermore, it was also successfully employed to determine HSA in biological samples. Thus, this method has potential applications in point-of-care medical diagnosis and biomedical research. View Full-Text
Keywords: label-free; human serum albumin; copper nanoparticles label-free; human serum albumin; copper nanoparticles

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Chen, M.; Xiang, X.; Wu, K.; He, H.; Chen, H.; Ma, C. A Novel Detection Method of Human Serum Albumin Based on the Poly(Thymine)-Templated Copper Nanoparticles. Sensors 2017, 17, 2684.

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