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Fluorescence Spectroscopy and Chemometric Modeling for Bioprocess Monitoring

Process Analytics and Cereal Science, Institute of Food Science and Biotechnology, University Hohenheim, Garbenstraße 23, 70599 Stuttgart, Germany
Author to whom correspondence should be addressed.
Academic Editor: Alexander Star
Sensors 2015, 15(5), 10271-10291;
Received: 27 February 2015 / Revised: 22 April 2015 / Accepted: 27 April 2015 / Published: 30 April 2015
(This article belongs to the Special Issue Sensors for Bioprocess Monitoring and Control)
On-line sensors for the detection of crucial process parameters are desirable for the monitoring, control and automation of processes in the biotechnology, food and pharma industry. Fluorescence spectroscopy as a highly developed and non-invasive technique that enables the on-line measurements of substrate and product concentrations or the identification of characteristic process states. During a cultivation process significant changes occur in the fluorescence spectra. By means of chemometric modeling, prediction models can be calculated and applied for process supervision and control to provide increased quality and the productivity of bioprocesses. A range of applications for different microorganisms and analytes has been proposed during the last years. This contribution provides an overview of different analysis methods for the measured fluorescence spectra and the model-building chemometric methods used for various microbial cultivations. Most of these processes are observed using the BioView® Sensor, thanks to its robustness and insensitivity to adverse process conditions. Beyond that, the PLS-method is the most frequently used chemometric method for the calculation of process models and prediction of process variables. View Full-Text
Keywords: fluorescence spectroscopy; chemometric modeling; sensors; biotechnology fluorescence spectroscopy; chemometric modeling; sensors; biotechnology
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Faassen, S.M.; Hitzmann, B. Fluorescence Spectroscopy and Chemometric Modeling for Bioprocess Monitoring. Sensors 2015, 15, 10271-10291.

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