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Diversity 2016, 8(1), 5;

Building a Plant DNA Barcode Reference Library for a Diverse Tropical Flora: An Example from Queensland, Australia

Botany Department, National Museum of Natural History, MRC 166, Smithsonian Institution, PO Box 37012, Washington, DC 20013-7012, USA
School of Biological Sciences and the Environment Institute, University of Adelaide, Adelaide 5005, Australia
National Herbarium of NSW, Royal Botanic Garden Sydney, Mrs Macquaries Road, Sydney 2000, Australia
Department of Biological Sciences, Macquarie University, Sydney 2109, Australia
CSIRO Land and Water, Tropical Forest Research Centre, Atherton 4883, Australia
Australian Tropical Herbarium, James Cook University, Smithfield 4878, Australia
These authors contributed equally to this work.
Author to whom correspondence should be addressed.
Academic Editor: Michael Wink
Received: 19 October 2015 / Revised: 14 February 2016 / Accepted: 24 February 2016 / Published: 29 February 2016
(This article belongs to the Special Issue DNA Barcoding and Biodiversity Research)
Full-Text   |   PDF [833 KB, uploaded 29 February 2016]   |  


A foundation for a DNA barcode reference library for the tropical plants of Australia is presented here. A total of 1572 DNA barcode sequences are compiled from 848 tropical Queensland species. The dataset represents 35% of the total flora of Queensland’s Wet Tropics Bioregion, 57% of its tree species and 28% of the shrub species. For approximately half of the sampled species, we investigated the occurrence of infraspecific molecular variation in DNA barcode loci rbcLa, matK, and the trnH-psbA intergenic spacer region across previously recognized biogeographic barriers. We found preliminary support for the notion that DNA barcode reference libraries can be used as a tool for inferring biogeographic patterns at regional scales. It is expected that this dataset will find applications in taxonomic, ecological, and applied conservation research. View Full-Text
Keywords: biogeography; wet tropics; rbcLa; matK; trnH-psbA intergenic spacer biogeography; wet tropics; rbcLa; matK; trnH-psbA intergenic spacer

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Costion, C.M.; Lowe, A.J.; Rossetto, M.; Kooyman, R.M.; Breed, M.F.; Ford, A.; Crayn, D.M. Building a Plant DNA Barcode Reference Library for a Diverse Tropical Flora: An Example from Queensland, Australia. Diversity 2016, 8, 5.

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