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Open AccessArticle

Building a Plant DNA Barcode Reference Library for a Diverse Tropical Flora: An Example from Queensland, Australia

1
Botany Department, National Museum of Natural History, MRC 166, Smithsonian Institution, PO Box 37012, Washington, DC 20013-7012, USA
2
School of Biological Sciences and the Environment Institute, University of Adelaide, Adelaide 5005, Australia
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National Herbarium of NSW, Royal Botanic Garden Sydney, Mrs Macquaries Road, Sydney 2000, Australia
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Department of Biological Sciences, Macquarie University, Sydney 2109, Australia
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CSIRO Land and Water, Tropical Forest Research Centre, Atherton 4883, Australia
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Australian Tropical Herbarium, James Cook University, Smithfield 4878, Australia
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editor: Michael Wink
Diversity 2016, 8(1), 5; https://doi.org/10.3390/d8010005
Received: 19 October 2015 / Revised: 14 February 2016 / Accepted: 24 February 2016 / Published: 29 February 2016
(This article belongs to the Special Issue DNA Barcoding and Biodiversity Research)
A foundation for a DNA barcode reference library for the tropical plants of Australia is presented here. A total of 1572 DNA barcode sequences are compiled from 848 tropical Queensland species. The dataset represents 35% of the total flora of Queensland’s Wet Tropics Bioregion, 57% of its tree species and 28% of the shrub species. For approximately half of the sampled species, we investigated the occurrence of infraspecific molecular variation in DNA barcode loci rbcLa, matK, and the trnH-psbA intergenic spacer region across previously recognized biogeographic barriers. We found preliminary support for the notion that DNA barcode reference libraries can be used as a tool for inferring biogeographic patterns at regional scales. It is expected that this dataset will find applications in taxonomic, ecological, and applied conservation research. View Full-Text
Keywords: biogeography; wet tropics; rbcLa; matK; trnH-psbA intergenic spacer biogeography; wet tropics; rbcLa; matK; trnH-psbA intergenic spacer
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MDPI and ACS Style

Costion, C.M.; Lowe, A.J.; Rossetto, M.; Kooyman, R.M.; Breed, M.F.; Ford, A.; Crayn, D.M. Building a Plant DNA Barcode Reference Library for a Diverse Tropical Flora: An Example from Queensland, Australia. Diversity 2016, 8, 5.

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