Lycorine Inhibits Influenza Virus Replication by Affecting Nascent Nucleoporin Nup93 Synthesis
Abstract
1. Introduction
2. Results
2.1. Lycorine Inhibits the Replication of Influenza Virus
2.2. Lycorine Affects the Multiple Stages of the Viral Replication Cycle Following Internalization
2.3. Lycorine Inhibits Nuclear Export of IAV NP
2.4. Lycorine Affects the Expression of Nup93
2.5. Lycorine Inhibits the Nascent Synthesis of Nup93 Protein
3. Discussion
4. Materials and Methods
4.1. Cell Culture and Virus
4.2. Compounds
4.3. Cytotoxicity Assay
4.4. Anti-IAV Assay
4.5. Western Blot
4.6. Real-Time Quantitative PCR Analysis
4.7. Virus Titer Determination
4.8. Time-of-Addition Assay and Time-Course Assay
4.9. Immunofluorescence
4.10. Nascent Protein Synthesis Assay
4.11. Statistical Analysis
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Genes | Sequence (5′-3′) |
---|---|
Influenza M2-F | GACCRATCCTGTCACCTCTGAC |
Influenza M2-R | GGGCATTYTGGACAAAKCGTCTACG |
Influenza HA-F | CAAACAGAAGACGGAGGACTACCAC |
Influenza HA-R | ATTACCTTGCTCCTGCCACTTGC |
GAPDH-F(H) | GGTGGTCTCCTCTGACTTCAACA |
GAPDH-R(H) | GTTGCTGTAGCCAAATTCGTTGT |
Gapdh-F(D) | AGTCAAGGCTGAGAACGGGAAACT |
Gapdh-R(D) | TCCACAACATACTCAGCACCAGCA |
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Yan, H.; Wang, H.; Wang, K.; Wu, S.; Jiang, J.; Li, Y. Lycorine Inhibits Influenza Virus Replication by Affecting Nascent Nucleoporin Nup93 Synthesis. Int. J. Mol. Sci. 2025, 26, 5358. https://doi.org/10.3390/ijms26115358
Yan H, Wang H, Wang K, Wu S, Jiang J, Li Y. Lycorine Inhibits Influenza Virus Replication by Affecting Nascent Nucleoporin Nup93 Synthesis. International Journal of Molecular Sciences. 2025; 26(11):5358. https://doi.org/10.3390/ijms26115358
Chicago/Turabian StyleYan, Haiyan, Huiqiang Wang, Kun Wang, Shuo Wu, Jiandong Jiang, and Yuhuan Li. 2025. "Lycorine Inhibits Influenza Virus Replication by Affecting Nascent Nucleoporin Nup93 Synthesis" International Journal of Molecular Sciences 26, no. 11: 5358. https://doi.org/10.3390/ijms26115358
APA StyleYan, H., Wang, H., Wang, K., Wu, S., Jiang, J., & Li, Y. (2025). Lycorine Inhibits Influenza Virus Replication by Affecting Nascent Nucleoporin Nup93 Synthesis. International Journal of Molecular Sciences, 26(11), 5358. https://doi.org/10.3390/ijms26115358