Metabolic Background, Not Photosynthetic Physiology, Determines Drought and Drought Recovery Responses in C3 and C2 Moricandias
Abstract
:1. Introduction
2. Results
2.1. Cross-Species Comparison under Control Conditions
2.1.1. Physiology
2.1.2. Biochemistry and Transcriptome Profiles
2.2. Progressive Soil Water Deficit and Rewatering
2.2.1. Physiological Evaluation
2.2.2. Cross-Species Comparison at the Physiological and Biochemical Levels
2.2.3. Cross-Species Transcriptome Comparison at T2 and RW
3. Discussion
4. Materials and Methods
4.1. Plant Material
4.2. Plant Growth Conditions
4.3. Drought Treatment and Leaf Sampling
4.4. Plant Gas Exchange
4.5. Biochemical Analysis
4.6. Estimation of the Reducing Capacity by the Folin–Ciocalteau Method
4.7. Extraction and Quantification of Water-Soluble Carbohydrates and Starch
4.8. RNA Isolation, RNA-Seq and Bioinformatic Analysis
4.9. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Pinheiro, C.; Emiliani, G.; Marino, G.; Fortunato, A.S.; Haworth, M.; De Carlo, A.; Chaves, M.M.; Loreto, F.; Centritto, M. Metabolic Background, Not Photosynthetic Physiology, Determines Drought and Drought Recovery Responses in C3 and C2 Moricandias. Int. J. Mol. Sci. 2023, 24, 4094. https://doi.org/10.3390/ijms24044094
Pinheiro C, Emiliani G, Marino G, Fortunato AS, Haworth M, De Carlo A, Chaves MM, Loreto F, Centritto M. Metabolic Background, Not Photosynthetic Physiology, Determines Drought and Drought Recovery Responses in C3 and C2 Moricandias. International Journal of Molecular Sciences. 2023; 24(4):4094. https://doi.org/10.3390/ijms24044094
Chicago/Turabian StylePinheiro, Carla, Giovanni Emiliani, Giovanni Marino, Ana S. Fortunato, Matthew Haworth, Anna De Carlo, Maria Manuela Chaves, Francesco Loreto, and Mauro Centritto. 2023. "Metabolic Background, Not Photosynthetic Physiology, Determines Drought and Drought Recovery Responses in C3 and C2 Moricandias" International Journal of Molecular Sciences 24, no. 4: 4094. https://doi.org/10.3390/ijms24044094