Protective Effects of Hexarelin and JMV2894 in a Human Neuroblastoma Cell Line Expressing the SOD1-G93A Mutated Protein
Abstract
:1. Introduction
2. Results
2.1. Dose-Response Study with H2O2 on Cell Viability
2.2. Dose–Response Study with GHS for the Treatment of SH-SY5Y SOD1G93A Cells
2.3. Effects of GHS on Morphological Changes Induced by H2O2-Treatment in SH-SY5Y SOD1G93A Cells
- 1-
- 2-
- 3-
- Maximum Span Across the Convex Hull (MSACH), which is the maximum distance between two points across the convex hull.
- 4-
- Perimeter, calculated as the number of pixels on the outlined cell shape.
- 5-
- Area, quantified as the total number of pixels present in the filled shape of the cell image.
2.4. H2O2-Induced Modulation of BCL-2 Family mRNA Levels in SH-SY5Y WT and SOD1G93A Cells
2.5. Effects of GHS on H2O2-Induced Modulation of BCL-2 Family mRNA Levels in SH-SY5Y SOD1G93A Cells
2.6. Effects of H2O2 on Caspases-3 and -7 mRNA Expressions
2.7. Effects of GHS on Caspases-3 and -7 mRNA Expressions and Protein Levels Induced by H2O2-Treatment
2.8. Effects of GHS on ERK 1/2, p38 and Akt Protein Levels in H2O2-Treated Cells
2.9. GHS Activity on γH2AX Phosphorylation
3. Discussion
4. Materials and Methods
4.1. Chemicals
4.2. Cell Culture
4.3. Cell Viability
4.4. Actin Staining Assay
4.5. Morphological Analysis
4.6. Real-Time PCR Analysis
4.7. Western Blot Analysis
4.8. The γH2AX Distribution
4.9. Statistical Analysis
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Meanti, R.; Licata, M.; Rizzi, L.; Bresciani, E.; Molteni, L.; Coco, S.; Locatelli, V.; Omeljaniuk, R.J.; Torsello, A. Protective Effects of Hexarelin and JMV2894 in a Human Neuroblastoma Cell Line Expressing the SOD1-G93A Mutated Protein. Int. J. Mol. Sci. 2023, 24, 993. https://doi.org/10.3390/ijms24020993
Meanti R, Licata M, Rizzi L, Bresciani E, Molteni L, Coco S, Locatelli V, Omeljaniuk RJ, Torsello A. Protective Effects of Hexarelin and JMV2894 in a Human Neuroblastoma Cell Line Expressing the SOD1-G93A Mutated Protein. International Journal of Molecular Sciences. 2023; 24(2):993. https://doi.org/10.3390/ijms24020993
Chicago/Turabian StyleMeanti, Ramona, Martina Licata, Laura Rizzi, Elena Bresciani, Laura Molteni, Silvia Coco, Vittorio Locatelli, Robert J. Omeljaniuk, and Antonio Torsello. 2023. "Protective Effects of Hexarelin and JMV2894 in a Human Neuroblastoma Cell Line Expressing the SOD1-G93A Mutated Protein" International Journal of Molecular Sciences 24, no. 2: 993. https://doi.org/10.3390/ijms24020993