Activation of Free Fatty Acid Receptor 4 (FFA4) Ameliorates Ovalbumin-Induced Allergic Asthma by Suppressing Activation of Dendritic and Mast Cells in Mice
Abstract
:1. Introduction
2. Results
2.1. Compound A Suppressed Degranulation of Mast Cells
2.2. Compound A Ameliorated Asthma-Induced Influx of Immune Cells in FFA4-WT Mice
2.3. Compound A Ameliorated Asthma-Induced Inflammation and Mucin Secretion in the Lungs of FFA4-WT Mice
2.4. Compound A Ameliorated Asthma-Induced Cytokine Expression in FFA4-WT Mice
2.5. Compound A Did Not Ameliorate Asthma-Induced Influx of Immune Cells in FFA4-KO Mice
2.6. Compound A Did Not Ameliorate Asthma-Induced Inflammation and Mucin Secretion in the Lungs of FFA4-KO Mice
2.7. Compound A Did Not Ameliorate Asthma-Induced Cytokine Expression in FFA4-KO Mice
2.8. Changes of Serum IgE and BALF Cytokine Levels by OVA and Compound A in Mice
3. Discussion
4. Materials and Methods
4.1. Materials
4.2. Animals
4.3. Cell Culture
4.4. Assessment of Degranulation
4.5. Asthma Induction in Mice and Administration of Compound A
4.6. Cell Counting and Analysis in BALF
4.7. Histological Examination of the Lungs
4.8. Quantitative Real-Time PCR
4.9. Statistical Analysis
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Son, S.-E.; Koh, J.-M.; Im, D.-S. Activation of Free Fatty Acid Receptor 4 (FFA4) Ameliorates Ovalbumin-Induced Allergic Asthma by Suppressing Activation of Dendritic and Mast Cells in Mice. Int. J. Mol. Sci. 2022, 23, 5270. https://doi.org/10.3390/ijms23095270
Son S-E, Koh J-M, Im D-S. Activation of Free Fatty Acid Receptor 4 (FFA4) Ameliorates Ovalbumin-Induced Allergic Asthma by Suppressing Activation of Dendritic and Mast Cells in Mice. International Journal of Molecular Sciences. 2022; 23(9):5270. https://doi.org/10.3390/ijms23095270
Chicago/Turabian StyleSon, So-Eun, Jung-Min Koh, and Dong-Soon Im. 2022. "Activation of Free Fatty Acid Receptor 4 (FFA4) Ameliorates Ovalbumin-Induced Allergic Asthma by Suppressing Activation of Dendritic and Mast Cells in Mice" International Journal of Molecular Sciences 23, no. 9: 5270. https://doi.org/10.3390/ijms23095270