The Family Keeps on Growing: Four Novel Fungal OYEs Characterized

Robescu, Marina Simona; Loprete, Giovanni; Gasparotto, Matteo; Vascon, Filippo; Filippini, Francesco; Cendron, Laura; Bergantino, Elisabetta

doi:10.3390/ijms23063050

Open AccessArticle

The Family Keeps on Growing: Four Novel Fungal OYEs Characterized

by

Marina Simona Robescu

^†

,

Giovanni Loprete

,

Matteo Gasparotto

,

Filippo Vascon

,

Francesco Filippini

,

Laura Cendron

and

Elisabetta Bergantino

^*

Synthetic Biology and Biotechnology Unit, Department of Biology, University of Padova, Viale G. Colombo 3, 35131 Padova, Italy

^*

Author to whom correspondence should be addressed.

^†

Current Address: Department of Drug Sciences, University of Pavia, Viale Taramelli 12, 27100 Pavia, Italy.

Int. J. Mol. Sci. 2022, 23(6), 3050; https://doi.org/10.3390/ijms23063050

Submission received: 31 January 2022 / Revised: 7 March 2022 / Accepted: 9 March 2022 / Published: 11 March 2022

(This article belongs to the Special Issue Enzymes as Targets for Drug Development II)

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Abstract

Aiming at expanding the portfolio of Old Yellow Enzymes (OYEs), which have been systematically studied to be employed in the chemical and pharmaceutical industries as useful biocatalysts, we decided to explore the immense reservoir of filamentous fungi. We drew from the genome of the two Ascomycetes Aspergillus niger and Botryotinia fuckeliana four new members of the OYE superfamily belonging to the classical and thermophilic-like subfamilies. The two BfOYEs show wider substrate spectra than the AnOYE homologues, which appear as more specialized biocatalysts. According to their mesophilic origins, the new enzymes neither show high thermostability nor extreme pH optimums. The crystal structures of BfOYE4 and AnOYE8 have been determined, revealing the conserved features of the thermophilic-like subclass as well as unique properties, such as a peculiar N-terminal loop involved in dimer surface interactions. For the classical representatives BfOYE1 and AnOYE2, model structures were built and analyzed, showing surprisingly wide open access to the active site cavities due to a shorter β6-loop and a disordered capping subdomain.

Keywords: old yellow enzyme; Aspergillus niger; Botryotinia fuckeliana; fungi; biocatalysis

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MDPI and ACS Style

Robescu, M.S.; Loprete, G.; Gasparotto, M.; Vascon, F.; Filippini, F.; Cendron, L.; Bergantino, E. The Family Keeps on Growing: Four Novel Fungal OYEs Characterized. Int. J. Mol. Sci. 2022, 23, 3050. https://doi.org/10.3390/ijms23063050

AMA Style

Robescu MS, Loprete G, Gasparotto M, Vascon F, Filippini F, Cendron L, Bergantino E. The Family Keeps on Growing: Four Novel Fungal OYEs Characterized. International Journal of Molecular Sciences. 2022; 23(6):3050. https://doi.org/10.3390/ijms23063050

Chicago/Turabian Style

Robescu, Marina Simona, Giovanni Loprete, Matteo Gasparotto, Filippo Vascon, Francesco Filippini, Laura Cendron, and Elisabetta Bergantino. 2022. "The Family Keeps on Growing: Four Novel Fungal OYEs Characterized" International Journal of Molecular Sciences 23, no. 6: 3050. https://doi.org/10.3390/ijms23063050

APA Style

Robescu, M. S., Loprete, G., Gasparotto, M., Vascon, F., Filippini, F., Cendron, L., & Bergantino, E. (2022). The Family Keeps on Growing: Four Novel Fungal OYEs Characterized. International Journal of Molecular Sciences, 23(6), 3050. https://doi.org/10.3390/ijms23063050

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The Family Keeps on Growing: Four Novel Fungal OYEs Characterized

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