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Article

Amino Acids and IGF1 Regulation of Fish Muscle Growth Revealed by Transcriptome and microRNAome Integrative Analyses of Pacu (Piaractus mesopotamicus) Myotubes

1
Department of Histology, Embryology and Cell Biology, Institute of Biological Sciences, Federal University of Goiás (UFG), Goiânia 74690-900, GO, Brazil
2
Department of Structural and Functional Biology, Institute of Biosciences, São Paulo State University (UNESP), Botucatu 18618-689, SP, Brazil
3
Environment and Regional Development Graduate Program, University of Western São Paulo (UNOESTE), Presidente Prudente 19050-680, SP, Brazil
4
Department of Cell Biology, Physiology and Immunology, Faculty of Biology, University of Barcelona, 08028 Barcelona, Spain
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editor: Vincenzo Sorrentino
Int. J. Mol. Sci. 2022, 23(3), 1180; https://doi.org/10.3390/ijms23031180
Received: 26 December 2021 / Revised: 12 January 2022 / Accepted: 17 January 2022 / Published: 21 January 2022
(This article belongs to the Special Issue Endocrine Control of Fish Metabolism)
Amino acids (AA) and IGF1 have been demonstrated to play essential roles in protein synthesis and fish muscle growth. The myoblast cell culture is useful for studying muscle regulation, and omics data have contributed enormously to understanding its molecular biology. However, to our knowledge, no study has performed the large-scale sequencing of fish-cultured muscle cells stimulated with pro-growth signals. In this work, we obtained the transcriptome and microRNAome of pacu (Piaractus mesopotamicus)-cultured myotubes treated with AA or IGF1. We identified 1228 and 534 genes differentially expressed by AA and IGF1. An enrichment analysis showed that AA treatment induced chromosomal changes, mitosis, and muscle differentiation, while IGF1 modulated IGF/PI3K signaling, metabolic alteration, and matrix structure. In addition, potential molecular markers were similarly modulated by both treatments. Muscle-miRNAs (miR-1, -133, -206 and -499) were up-regulated, especially in AA samples, and we identified molecular networks with omics integration. Two pairs of genes and miRNAs demonstrated a high-level relationship, and involvement in myogenesis and muscle growth: marcksb and miR-29b in AA, and mmp14b and miR-338-5p in IGF1. Our work helps to elucidate fish muscle physiology and metabolism, highlights potential molecular markers, and creates a perspective for improvements in aquaculture and in in vitro meat production. View Full-Text
Keywords: muscle growth; cell culture; amino acids; IGF1; omics muscle growth; cell culture; amino acids; IGF1; omics
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MDPI and ACS Style

Duran, B.O.S.; Zanella, B.T.T.; Perez, E.S.; Mareco, E.A.; Blasco, J.; Dal-Pai-Silva, M.; Garcia de la serrana, D. Amino Acids and IGF1 Regulation of Fish Muscle Growth Revealed by Transcriptome and microRNAome Integrative Analyses of Pacu (Piaractus mesopotamicus) Myotubes. Int. J. Mol. Sci. 2022, 23, 1180. https://doi.org/10.3390/ijms23031180

AMA Style

Duran BOS, Zanella BTT, Perez ES, Mareco EA, Blasco J, Dal-Pai-Silva M, Garcia de la serrana D. Amino Acids and IGF1 Regulation of Fish Muscle Growth Revealed by Transcriptome and microRNAome Integrative Analyses of Pacu (Piaractus mesopotamicus) Myotubes. International Journal of Molecular Sciences. 2022; 23(3):1180. https://doi.org/10.3390/ijms23031180

Chicago/Turabian Style

Duran, Bruno O.S., Bruna T.T. Zanella, Erika S. Perez, Edson A. Mareco, Josefina Blasco, Maeli Dal-Pai-Silva, and Daniel Garcia de la serrana. 2022. "Amino Acids and IGF1 Regulation of Fish Muscle Growth Revealed by Transcriptome and microRNAome Integrative Analyses of Pacu (Piaractus mesopotamicus) Myotubes" International Journal of Molecular Sciences 23, no. 3: 1180. https://doi.org/10.3390/ijms23031180

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