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Article

Validated Growth Rate-Dependent Regulation of Lipid Metabolism in Yarrowia lipolytica

1
Department of Biology and Biological Engineering, Chalmers University of Technology, SE-412 96 Gothenburg, Sweden
2
Novo Nordisk Foundation Center for Biosustainability, Chalmers University of Technology, SE-412 96 Gothenburg, Sweden
*
Author to whom correspondence should be addressed.
Academic Editor: Jean-Marc Nicaud
Int. J. Mol. Sci. 2022, 23(15), 8517; https://doi.org/10.3390/ijms23158517
Received: 12 July 2022 / Revised: 26 July 2022 / Accepted: 29 July 2022 / Published: 31 July 2022
Given the strong potential of Yarrowia lipolytica to produce lipids for use as renewable fuels and oleochemicals, it is important to gain in-depth understanding of the molecular mechanism underlying its lipid accumulation. As cellular growth rate affects biomass lipid content, we performed a comparative proteomic analysis of Y. lipolytica grown in nitrogen-limited chemostat cultures at different dilution rates. After confirming the correlation between growth rate and lipid accumulation, we were able to identify various cellular functions and biological mechanisms involved in oleaginousness. Inspection of significantly up- and downregulated proteins revealed nonintuitive processes associated with lipid accumulation in this yeast. This included proteins related to endoplasmic reticulum (ER) stress, ER–plasma membrane tether proteins, and arginase. Genetic engineering of selected targets validated that some genes indeed affected lipid accumulation. They were able to increase lipid content and were complementary to other genetic engineering strategies to optimize lipid yield. View Full-Text
Keywords: lipid accumulation; Yarrowia lipolytica; comparative proteomics; oleaginous yeast lipid accumulation; Yarrowia lipolytica; comparative proteomics; oleaginous yeast
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MDPI and ACS Style

Poorinmohammad, N.; Fu, J.; Wabeke, B.; Kerkhoven, E.J. Validated Growth Rate-Dependent Regulation of Lipid Metabolism in Yarrowia lipolytica. Int. J. Mol. Sci. 2022, 23, 8517. https://doi.org/10.3390/ijms23158517

AMA Style

Poorinmohammad N, Fu J, Wabeke B, Kerkhoven EJ. Validated Growth Rate-Dependent Regulation of Lipid Metabolism in Yarrowia lipolytica. International Journal of Molecular Sciences. 2022; 23(15):8517. https://doi.org/10.3390/ijms23158517

Chicago/Turabian Style

Poorinmohammad, Naghmeh, Jing Fu, Bob Wabeke, and Eduard J. Kerkhoven. 2022. "Validated Growth Rate-Dependent Regulation of Lipid Metabolism in Yarrowia lipolytica" International Journal of Molecular Sciences 23, no. 15: 8517. https://doi.org/10.3390/ijms23158517

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