The Anticancer Activity Conferred by the Mud Crab Antimicrobial Peptide Scyreprocin through Apoptosis and Membrane Disruption

State-Province Joint Engineering Laboratory of Marine Bioproducts and Technology, College of Ocean and Earth Sciences, Xiamen University, Xiamen 361102, China

Fujian Innovation Research Institute for Marine Biological Antimicrobial Peptide Industrial Technology, College of Ocean & Earth Sciences, Xiamen University, Xiamen 361102, China

Author to whom correspondence should be addressed.

Academic Editor: Giovanna Batoni

Int. J. Mol. Sci. 2022, 23(10), 5500; https://doi.org/10.3390/ijms23105500

Received: 17 April 2022 / Revised: 4 May 2022 / Accepted: 11 May 2022 / Published: 14 May 2022

(This article belongs to the Special Issue Antibacterial Strategies in Biomaterials: Current Progress and Challenges)

View Full-Text Download PDF

Browse Figures

Review Reports Citation Export

Abstract

Scyreprocin is an antimicrobial peptide first identified in the mud crab Scylla paramamosain. Herein, we showed that its recombinant product (rScyreprocin) could significantly inhibit the growth of human lung cancer NCI-H460 cells (H460), but showed no cytotoxicity to human lung fibroblasts (HFL1). rScyreprocin was a membrane-active peptide that firstly induced the generation of reactive oxygen species (ROS) in H460, and led to endoplasmic reticulum stress and Ca²⁺ release, which resulted in mitochondrial dysfunction and subsequently activation of caspase-3 cascades, and ultimately led to apoptosis. The comprehensive results indicated that rScyreprocin exerted anticancer activity by disrupting cell membrane and inducing apoptosis. The in vivo efficacy test demonstrated that intratumoral injection of rScyreprocin significantly inhibited the growth of H460 xenografts, which was close to that of the cisplatin (inhibition rate: 69.94% vs. 80.76%). Therefore, rScyreprocin is expected to become a promising candidate for the treatment of lung cancer. View Full-Text

Keywords: scyreprocin; antimicrobial peptide; apoptosis; anti-tumor; non-small-cell lung cancer

▼ Show Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited

Supplementary Material

Supplementary File 1:
ZIP-Document (ZIP, 1265 KiB)

Share and Cite

MDPI and ACS Style

Yang, Y.; Chen, H.-Y.; Hao, H.; Wang, K.-J. The Anticancer Activity Conferred by the Mud Crab Antimicrobial Peptide Scyreprocin through Apoptosis and Membrane Disruption. Int. J. Mol. Sci. 2022, 23, 5500. https://doi.org/10.3390/ijms23105500

AMA Style

Yang Y, Chen H-Y, Hao H, Wang K-J. The Anticancer Activity Conferred by the Mud Crab Antimicrobial Peptide Scyreprocin through Apoptosis and Membrane Disruption. International Journal of Molecular Sciences. 2022; 23(10):5500. https://doi.org/10.3390/ijms23105500

Chicago/Turabian Style

Yang, Ying, Hui-Yun Chen, Hua Hao, and Ke-Jian Wang. 2022. "The Anticancer Activity Conferred by the Mud Crab Antimicrobial Peptide Scyreprocin through Apoptosis and Membrane Disruption" International Journal of Molecular Sciences 23, no. 10: 5500. https://doi.org/10.3390/ijms23105500

Find Other Styles

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Metrics

Abstract views Pdf views Html views

Article Menu

The Anticancer Activity Conferred by the Mud Crab Antimicrobial Peptide Scyreprocin through Apoptosis and Membrane Disruption

Abstract

Supplementary Material

Share and Cite

Article Metrics

Article Access Statistics

Article Access Map by Country/Region

Further Information

Guidelines

MDPI Initiatives

Follow MDPI