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Cellular Response to Individual Components of the Platelet Concentrate

Department of Biophysics, 2nd Faculty of Medicine, Charles University, V Uvalu 84, 150 06 Prague, Czech Republic
Department of Tissue Engineering, Institute of Experimental Medicine, The Czech Academy of Sciences, Videnska 1083, 142 20 Prague, Czech Republic
University Centre for Energy Efficient Buildings, Czech Technical University in Prague, Trinecka 1024, 273 43 Bustehrad, Czech Republic
Author to whom correspondence should be addressed.
Academic Editor: Valeria Gasperi
Int. J. Mol. Sci. 2021, 22(9), 4539;
Received: 24 March 2021 / Revised: 23 April 2021 / Accepted: 23 April 2021 / Published: 26 April 2021
(This article belongs to the Special Issue Molecular Research on Platelet Activity in Health and Disease 3.0)
Platelet concentrates and especially their further product platelet lysate, are widely used as a replacement for cell culturing. Platelets contain a broad spectrum of growth factors and bioactive molecules that affect cellular fate. However, the cellular response to individual components of the human platelet concentrate is still unclear. The aim of this study was to observe cellular behavior according to the individual components of platelet concentrates. The bioactive molecule content was determined. The cells were supplemented with a medium containing 8% (v/v) of platelet proteins in plasma, pure platelet proteins in deionized water, and pure plasma. The results showed a higher concentration of fibrinogen, albumin, insulin growth factor I (IGF-1), keratinocyte growth factor (KGF), and hepatocyte growth factor (HGF), in the groups containing plasma. On the other hand, chemokine RANTES and platelet-derived growth factor bb (PDGF-bb), were higher in the groups containing platelet proteins. The groups containing both plasma and plasma proteins showed the most pronounced proliferation and viability of mesenchymal stem cells and fibroblasts. The platelet proteins alone were not sufficient to provide optimal cell growth and viability. A synergic effect of platelet proteins and plasma was observed. The data indicated the importance of plasma in platelet lysate for cell growth. View Full-Text
Keywords: platelets; plasma; mesenchymal stem cells; fibroblasts platelets; plasma; mesenchymal stem cells; fibroblasts
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MDPI and ACS Style

Sovkova, V.; Vocetkova, K.; Hedvičáková, V.; Hefka Blahnová, V.; Buzgo, M.; Amler, E.; Filová, E. Cellular Response to Individual Components of the Platelet Concentrate. Int. J. Mol. Sci. 2021, 22, 4539.

AMA Style

Sovkova V, Vocetkova K, Hedvičáková V, Hefka Blahnová V, Buzgo M, Amler E, Filová E. Cellular Response to Individual Components of the Platelet Concentrate. International Journal of Molecular Sciences. 2021; 22(9):4539.

Chicago/Turabian Style

Sovkova, Vera, Karolina Vocetkova, Věra Hedvičáková, Veronika Hefka Blahnová, Matěj Buzgo, Evzen Amler, and Eva Filová. 2021. "Cellular Response to Individual Components of the Platelet Concentrate" International Journal of Molecular Sciences 22, no. 9: 4539.

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