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Article

Latently KSHV-Infected Cells Promote Further Establishment of Latency upon Superinfection with KSHV

1
The Mina and Everard Goodman Faculty of Life Sciences, Bar-Ilan University, Ramat Gan 5290002, Israel
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Advanced Materials and Nanotechnology Institute, Bar-Ilan University, Ramat Gan 5290002, Israel
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Daniella Lee Casper Laboratory in Viral Oncology, Azrieli Faculty of Medicine, Bar-Ilan University, Safed 1311502, Israel
*
Author to whom correspondence should be addressed.
Academic Editor: Philippe Desprès
Int. J. Mol. Sci. 2021, 22(21), 11994; https://doi.org/10.3390/ijms222111994
Received: 9 October 2021 / Revised: 27 October 2021 / Accepted: 3 November 2021 / Published: 5 November 2021
(This article belongs to the Special Issue Herpesviruses and Their Host Cells: EBV- and KSHV-Associated Diseases)
Kaposi’s sarcoma-associated herpesvirus (KSHV) is a cancer-related virus which engages in two forms of infection: latent and lytic. Latent infection allows the virus to establish long-term persistent infection, whereas the lytic cycle is needed for the maintenance of the viral reservoir and for virus spread. By using recombinant KSHV viruses encoding mNeonGreen and mCherry fluorescent proteins, we show that various cell types that are latently-infected with KSHV can be superinfected, and that the new incoming viruses establish latent infection. Moreover, we show that latency establishment is enhanced in superinfected cells compared to primary infected ones. Further analysis revealed that cells that ectopically express the major latency protein of KSHV, LANA-1, prior to and during infection exhibit enhanced establishment of latency, but not cells expressing LANA-1 fragments. This observation supports the notion that the expression level of LANA-1 following infection determines the efficiency of latency establishment and avoids loss of viral genomes. These findings imply that a host can be infected with more than a single viral genome and that superinfection may support the maintenance of long-term latency. View Full-Text
Keywords: Kaposi’s sarcoma-associated herpesvirus (KSHV); superinfection; primary infection; latency-associated nuclear antigen 1 (LANA-1); latent infection Kaposi’s sarcoma-associated herpesvirus (KSHV); superinfection; primary infection; latency-associated nuclear antigen 1 (LANA-1); latent infection
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MDPI and ACS Style

Gam ze Letova, C.; Kalt, I.; Shamay, M.; Sarid, R. Latently KSHV-Infected Cells Promote Further Establishment of Latency upon Superinfection with KSHV. Int. J. Mol. Sci. 2021, 22, 11994. https://doi.org/10.3390/ijms222111994

AMA Style

Gam ze Letova C, Kalt I, Shamay M, Sarid R. Latently KSHV-Infected Cells Promote Further Establishment of Latency upon Superinfection with KSHV. International Journal of Molecular Sciences. 2021; 22(21):11994. https://doi.org/10.3390/ijms222111994

Chicago/Turabian Style

Gam ze Letova, Chen, Inna Kalt, Meir Shamay, and Ronit Sarid. 2021. "Latently KSHV-Infected Cells Promote Further Establishment of Latency upon Superinfection with KSHV" International Journal of Molecular Sciences 22, no. 21: 11994. https://doi.org/10.3390/ijms222111994

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