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Article

Hierarchically Structured Polystyrene-Based Surfaces Amplifying Fluorescence Signals: Cytocompatibility with Human Induced Pluripotent Stem Cell

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Centre of Polymer Systems, Tomas Bata University in Zlin, 760 01 Zlin, Czech Republic
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Faculty of Science, Masaryk University, 625 00 Brno, Czech Republic
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Faculty of Technology, Tomas Bata University in Zlin, 760 01 Zlin, Czech Republic
*
Authors to whom correspondence should be addressed.
Academic Editors: Silvia Panseri, Monica Montesi and John G. Hardy
Int. J. Mol. Sci. 2021, 22(21), 11943; https://doi.org/10.3390/ijms222111943
Received: 9 September 2021 / Revised: 10 October 2021 / Accepted: 27 October 2021 / Published: 4 November 2021
(This article belongs to the Special Issue Cell-Biomaterial Interaction 2021)
An innovative multi-step phase separation process was used to prepare tissue culture for the polystyrene-based, hierarchically structured substrates, which mimicked in vivo microenvironment and architecture. Macro- (pore area from 3000 to 18,000 µm2; roughness (Ra) 7.2 ± 0.1 µm) and meso- (pore area from 50 to 300 µm2; Ra 1.1 ± 0.1 µm) structured substrates covered with micro-pores (area around 3 µm2) were prepared and characterised. Both types of substrate were suitable for human-induced pluripotent stem cell (hiPSC) cultivation and were found to be beneficial for the induction of cardiomyogenesis in hiPSC. This was confirmed both by the number of promoted proliferated cells and the expressions of specific markers (Nkx2.5, MYH6, MYL2, and MYL7). Moreover, the substrates amplified the fluorescence signal when Ca2+ flow was monitored. This property, together with cytocompatibility, make this material especially suitable for in vitro studies of cell/material interactions within tissue-mimicking environments. View Full-Text
Keywords: biomimetic; surfaces; human-induced pluripotent stem cells; fluorescence signal; cardiomyogenesis biomimetic; surfaces; human-induced pluripotent stem cells; fluorescence signal; cardiomyogenesis
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MDPI and ACS Style

Skopalová, K.; Radaszkiewicz, K.A.; Kadlečková, M.; Pacherník, J.; Minařík, A.; Capáková, Z.; Kašpárková, V.; Mráček, A.; Daďová, E.; Humpolíček, P. Hierarchically Structured Polystyrene-Based Surfaces Amplifying Fluorescence Signals: Cytocompatibility with Human Induced Pluripotent Stem Cell. Int. J. Mol. Sci. 2021, 22, 11943. https://doi.org/10.3390/ijms222111943

AMA Style

Skopalová K, Radaszkiewicz KA, Kadlečková M, Pacherník J, Minařík A, Capáková Z, Kašpárková V, Mráček A, Daďová E, Humpolíček P. Hierarchically Structured Polystyrene-Based Surfaces Amplifying Fluorescence Signals: Cytocompatibility with Human Induced Pluripotent Stem Cell. International Journal of Molecular Sciences. 2021; 22(21):11943. https://doi.org/10.3390/ijms222111943

Chicago/Turabian Style

Skopalová, Kateřina, Katarzyna A. Radaszkiewicz, Markéta Kadlečková, Jiří Pacherník, Antonín Minařík, Zdenka Capáková, Věra Kašpárková, Aleš Mráček, Eliška Daďová, and Petr Humpolíček. 2021. "Hierarchically Structured Polystyrene-Based Surfaces Amplifying Fluorescence Signals: Cytocompatibility with Human Induced Pluripotent Stem Cell" International Journal of Molecular Sciences 22, no. 21: 11943. https://doi.org/10.3390/ijms222111943

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