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Article

Non-Targeted Metabolite Profiling Reveals Host Metabolomic Reprogramming during the Interaction of Black Pepper with Phytophthora capsici

1
Transdisciplinary Biology, Rajiv Gandhi Centre for Biotechnology, Poojappura, Thiruvananthapuram 695014, India
2
Plant Cytogenetics and Molecular Biology Group, Institute of Biology, Biotechnology and Environmental Protection, Faculty of Natural Sciences, University of Silesia in Katowice, 40-032 Katowice, Poland
3
Institute of Biological, Environmental and Rural Studies, Aberystwyth University, Ceredigion SY23 3EE, UK
*
Authors to whom correspondence should be addressed.
Academic Editor: Mohammad Golam Mostofa
Int. J. Mol. Sci. 2021, 22(21), 11433; https://doi.org/10.3390/ijms222111433
Received: 26 August 2021 / Revised: 28 September 2021 / Accepted: 6 October 2021 / Published: 22 October 2021
(This article belongs to the Section Molecular Plant Sciences)
Phytophthora capsici is one of the most destructive pathogens causing quick wilt (foot rot) disease in black pepper (Piper nigrum L.) to which no effective resistance has been defined. To better understand the P. nigrumP. capsici pathosystem, we employed metabolomic approaches based on flow-infusion electrospray–high-resolution mass spectrometry. Changes in the leaf metabolome were assessed in infected and systemic tissues at 24 and 48 hpi. Principal Component Analysis of the derived data indicated that the infected leaves showed a rapid metabolic response by 24 hpi whereas the systemic leaves took 48 hpi to respond to the infection. The major sources of variations between infected leaf and systemic leaf were identified, and enrichment pathway analysis indicated, major shifts in amino acid, tricarboxylic acid cycle, nucleotide and vitamin B6 metabolism upon infection. Moreover, the individual metabolites involved in defensive phytohormone signalling were identified. RT-qPCR analysis of key salicylate and jasmonate biosynthetic genes indicated a transient reduction of expression at 24 hpi but this increased subsequently. Exogenous application of jasmonate and salicylate reduced P. capsici disease symptoms, but this effect was suppressed with the co-application of abscisic acid. The results are consistent with abscisic acid reprogramming, salicylate and jasmonate defences in infected leaves to facilitate the formation of disease. The augmentation of salicylate and jasmonate defences could represent an approach through which quick wilt disease could be controlled in black pepper. View Full-Text
Keywords: Piper nigrum; quick wilt; metabolomics; abscisic acid; salicylic acid; jasmonic acid Piper nigrum; quick wilt; metabolomics; abscisic acid; salicylic acid; jasmonic acid
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MDPI and ACS Style

Kattupalli, D.; Pinski, A.; Sreekumar, S.; Usha, A.; Girija, A.; Beckmann, M.; Mur, L.A.J.; Eppurathu Vasudevan, S. Non-Targeted Metabolite Profiling Reveals Host Metabolomic Reprogramming during the Interaction of Black Pepper with Phytophthora capsici. Int. J. Mol. Sci. 2021, 22, 11433. https://doi.org/10.3390/ijms222111433

AMA Style

Kattupalli D, Pinski A, Sreekumar S, Usha A, Girija A, Beckmann M, Mur LAJ, Eppurathu Vasudevan S. Non-Targeted Metabolite Profiling Reveals Host Metabolomic Reprogramming during the Interaction of Black Pepper with Phytophthora capsici. International Journal of Molecular Sciences. 2021; 22(21):11433. https://doi.org/10.3390/ijms222111433

Chicago/Turabian Style

Kattupalli, Divya, Artur Pinski, Sweda Sreekumar, Aswathi Usha, Aiswarya Girija, Manfred Beckmann, Luis A.J. Mur, and Soniya Eppurathu Vasudevan. 2021. "Non-Targeted Metabolite Profiling Reveals Host Metabolomic Reprogramming during the Interaction of Black Pepper with Phytophthora capsici" International Journal of Molecular Sciences 22, no. 21: 11433. https://doi.org/10.3390/ijms222111433

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