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Article

The Grapevine E3 Ubiquitin Ligase VriATL156 Confers Resistance against the Downy Mildew Pathogen Plasmopara viticola

1
Department of Biotechnology, University of Verona, Strada Le Grazie 15, CV1, 37134 Verona, Italy
2
Department of Pharmaceutical Sciences, University of Perugia, Borgo XX Giugno 72, 06121 Perugia, Italy
3
Institute for Sustainable Plant Protection, National Research Council (IPSP-CNR), Strada delle Cacce 73, 10135 Torino, Italy
*
Authors to whom correspondence should be addressed.
Current address: Institut für Mikrobiologie und Weinforschung, Johannes Gutenberg-Universität Mainz, 55128 Mainz, Germany.
Int. J. Mol. Sci. 2021, 22(2), 940; https://doi.org/10.3390/ijms22020940
Received: 23 December 2020 / Revised: 11 January 2021 / Accepted: 14 January 2021 / Published: 19 January 2021
Downy mildew, caused by Plasmopara viticola, is one of the most severe diseases of grapevine (Vitis vinifera L.). Genetic resistance is an effective and sustainable control strategy, but major resistance genes (encoding receptors for specific pathogen effectors) introgressed from wild Vitis species, although effective, may be non-durable because the pathogen can evolve to avoid specific recognition. Previous transcriptomic studies in the resistant species Vitis riparia highlighted the activation of signal transduction components during infection. The transfer of such components to V. vinifera might confer less specific and therefore more durable resistance. Here, we describe the generation of transgenic V. vinifera lines constitutively expressing the V. riparia E3 ubiquitin ligase gene VriATL156. Phenotypic and molecular analysis revealed that the transgenic plants were less susceptible to P. viticola than vector-only controls, confirming the role of this E3 ubiquitin ligase in the innate immune response. Two independent transgenic lines were selected for detailed analysis of the resistance phenotype by RNA-Seq and microscopy, revealing the profound reprogramming of transcription to achieve resistance that operates from the earliest stages of pathogen infection. The introduction of VriATL156 into elite grapevine cultivars could therefore provide an effective and sustainable control measure against downy mildew. View Full-Text
Keywords: ATL E3 ubiquitin ligases; plant functional genomics; promoter analysis; plant resistance; sustainable agriculture ATL E3 ubiquitin ligases; plant functional genomics; promoter analysis; plant resistance; sustainable agriculture
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MDPI and ACS Style

Vandelle, E.; Ariani, P.; Regaiolo, A.; Danzi, D.; Lovato, A.; Zadra, C.; Vitulo, N.; Gambino, G.; Polverari, A. The Grapevine E3 Ubiquitin Ligase VriATL156 Confers Resistance against the Downy Mildew Pathogen Plasmopara viticola. Int. J. Mol. Sci. 2021, 22, 940. https://doi.org/10.3390/ijms22020940

AMA Style

Vandelle E, Ariani P, Regaiolo A, Danzi D, Lovato A, Zadra C, Vitulo N, Gambino G, Polverari A. The Grapevine E3 Ubiquitin Ligase VriATL156 Confers Resistance against the Downy Mildew Pathogen Plasmopara viticola. International Journal of Molecular Sciences. 2021; 22(2):940. https://doi.org/10.3390/ijms22020940

Chicago/Turabian Style

Vandelle, Elodie, Pietro Ariani, Alice Regaiolo, Davide Danzi, Arianna Lovato, Claudia Zadra, Nicola Vitulo, Giorgio Gambino, and Annalisa Polverari. 2021. "The Grapevine E3 Ubiquitin Ligase VriATL156 Confers Resistance against the Downy Mildew Pathogen Plasmopara viticola" International Journal of Molecular Sciences 22, no. 2: 940. https://doi.org/10.3390/ijms22020940

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